MRM1 (NM_024864) Human Over-expression Lysate

Lysate of MRM1 over-expression cell lines, WB control

Transient overexpression lysate of mitochondrial rRNA methyltransferase 1 homolog (S. cerevisiae) (MRM1), nuclear gene encoding mitochondrial protein

Product Info Summary

SKU: LS079922
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of mitochondrial rRNA methyltransferase 1 homolog (S. cerevisiae) (MRM1), nuclear gene encoding mitochondrial protein

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Product Name

MRM1 (NM_024864) Human Over-expression Lysate

See all MRM1 products

SKU/Catalog Number

LS079922

Size

100 µg

Description

Transient overexpression lysate of mitochondrial rRNA methyltransferase 1 homolog (S. cerevisiae) (MRM1), nuclear gene encoding mitochondrial protein

Storage & Handling

Cite This Product

MRM1 (NM_024864) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS079922)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for MRM1 (Source: Uniprot.org, NCBI)

Uniprot ID

Q6IN84

Gene ID

79922

Gene Name

MRM1

Full Name

rRNA methyltransferase 1, mitochondrial

Weight

38.638kDa

Superfamily

class IV-like SAM-binding methyltransferase superfamily

Alternative Names

EC 2.1.1.-; FLJ22578; Mitochondrial large ribosomal RNA ribose methylase; mitochondrial rRNA methyltransferase 1 homolog (S. cerevisiae); rRNA methyltransferase 1, mitochondrial MRM1 mitochondrial rRNA methyltransferase 1 rRNA methyltransferase 1, mitochondrial|16S rRNA (guanosine(1145)-2-O)-methyltransferase|16S rRNA [Gm1145] 2-O-methyltransferase|CTB-75G16.3|mitochondrial large ribosomal RNA ribose methylase|mitochondrial rRNA methyltransferase 1 homolog

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on MRM1, check out the MRM1 Infographic

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