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Product Info Summary
|Description:||Transient overexpression lysate of KIAA1826 (KIAA1826)|
MSANTD4 (NM_032424) Human Over-expression Lysate
Transient overexpression lysate of KIAA1826 (KIAA1826)
Storage & Handling
Cite This Product
MSANTD4 (NM_032424) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084437)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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MSANTD4 Human Over-expression Lysates NM_032424.
Western blot validation of overexpression lysate (LS084437) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with MSANTD4 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for MSANTD4 (Source: Uniprot.org, NCBI)
Myb/SANT-like DNA-binding domain-containing protein 4
hypothetical protein LOC84437; KIAA1826 MSANTD4 KIAA1826 Myb/SANT DNA binding domain containing 4 with coiled-coils myb/SANT-like DNA-binding domain-containing protein 4|Myb/SANT-like DNA-binding domain containing 4 with coiled-coils*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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