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Product Info Summary
|Description:||Transient overexpression lysate of N(alpha)-acetyltransferase 25, NatB auxiliary subunit (NAA25)|
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NAP1 (NAA25) (NM_024953) Human Over-expression Lysate
Transient overexpression lysate of N(alpha)-acetyltransferase 25, NatB auxiliary subunit (NAA25)
Storage & Handling
Cite This Product
NAP1 (NAA25) (NM_024953) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS080018)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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NAP1 (NAA25) Human Over-expression Lysates NM_024953.
Western blot validation of overexpression lysate (LS080018) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with NAA25 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for NAA25 (Source: Uniprot.org, NCBI)
N-alpha-acetyltransferase 25, NatB auxiliary subunit
C12orf30DKFZp667K2112; chromosome 12 open reading frame 30; FLJ13089; MDM20mitochondrial distribution and morphology 20; Mitochondrial distribution and morphology protein 20; N(alpha)-acetyltransferase 25, NatB auxiliary subunit; N-alpha-acetyltransferase 25, NatB auxiliary subunit; NAP1; NatB complex subunit MDM20; N-terminal acetyltransferase B complex subunit MDM20; N-terminal acetyltransferase B complex subunit NAA25; p120 NAA25 C12orf30, MDM20, NAP1 N-alpha-acetyltransferase 25, NatB auxiliary subunit N-alpha-acetyltransferase 25, NatB auxiliary subunit|N-terminal acetyltransferase B complex subunit NAA25|mitochondrial distribution and morphology 20|natB complex subunit MDM20*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on NAA25, check out the NAA25 Infographic
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In this infographic, you will see the following information for NAA25: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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