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Keywords: immunohistochemistry controls, IHC experiment design, control types
In the lab, researchers invest time and effort to optimize the sample preparation and sample staining processes of IHC. When successful, the results produce a strong and specific signal. However, how do we know the results have been interpreted correctly? When designing your IHC experiment, it is crucial to include positive and negative controls. The controls will help verify whether or not the IHC staining results observed are accurate and valid while exposing experimental artefacts. Read on to find out the 6 established controls for IHC!
Keywords: IHC optimization tips, antigen retrieval principle explanation, immunohistochemistry protocol optimize, how to perform HIER antigen retrieval for IHC experiment
If you are performing IHC experiments, you may need to optimize your antigen retrieval methods. Techniques generally fall into two main categories: protease-induced epitope retrieval (PIER) and heat-induced epitope retrieval (HIER). In general, HIER has a much higher success rate and is recommended over PIER methods. The protocol for HIER must be optimized for each sample tissue, fixation method, and antigen. This method is especially time-, temperature-, buffer-, and pH-sensitive, and the best condition must be determined empirically. Here is a step-wise protocol that may be helpful for your immunohistochemistry experiment.
Keywords: IHC, immunohistochemistry, heat induced epitope retrieval, proteolytic induced epitope retrieval, PIER, HIER, antigen retrieval optimization
Antigen retrieval is often necessary when performing IHC experiments. Here are some key points to know about HIER and PIER methods of antigen retrieval. This protocol step needs to be optimized for every antigen of interest.
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Troubleshooting GuidesCD40 can be found on several cell types, such as B cells, macrophages, dendritic cells, and fibroblasts, demonstrating its critical role in immune response and host defense. Furthermore, their research suggests that antibodies against SARS-CoV spike ...
See MoreAfter saturation, the inactivated antibody replaces the primary antibody in the IHC protocol for the control. The samples are incubated with only the antibody diluent without adding the primary antibody. No Primary Controls No primary controls (aka s...
See MoreTroubleshooting guides 6 IHC Controls You Should Know In the lab, researchers invest time and effort to optimize the sample preparation and sample staining processes of IHC. Our antibodies are validated using WB, IHC, and flow cytometry against a pan...
See MoreNon-Aldehyde Fixatives In cases where aldehyde or precipitating fixatives are not optimal choices for the sample type or antigen, non-aldehyde fixatives have been used as alternatives. Diimidoester fixation: This fixation method utilizes dimethyl sub...
See MoreThese small proteins are produced by a broad range of cells, which include macrophages, B-Cells, T-Cells, basophils, mast cells, etc. On the surface of T cells are PD-1 (programmed cell death-1) receptors whose ligands include PD-L1 and PD-L2. When t...
See MoreFACS Sorting Preparation Checklist Mar 20 2017 Keywords: check list, technical tips, FACS, Flow cytometry, cell sorting preparation Are you preparing for a FACS experiment? 6 Helpful Tips FACS Multicolor Panel Design Oct 27 2017 Keywords: flow cytome...
See More*Note to educators: you are permitted to share BosterBio's resources and PDFs on your class websites and lab websites. Greetings Earthling, Are you familiar with the multiple methods you could use to perform an ELISA? Check our online catalog of comp...
See MoreAbout the Campaign World Cancer Day Homepage American Cancer Society UICC Center for Cancer Research Studying Cancer ? The American Cancer Society estimated 1,762,450 new cancer cases and 606,880 cancer deaths will happen in 2019 in the US. Click bel...
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