Troubleshooting guides

Troubleshooting guides

Download troubleshooting
handbooks for IHC, Western
blot and ELISA for FREE.

*Note to educators: you are permitted to share BosterBio's resources and PDFs on your class websites and lab websites. Please make sure to cite or link to the origin.

Which ELISA Is For You?

Greetings Earthling,

Are you familiar with the multiple methods you could use to perform an ELISA? Among the standard assay formats illustrated below, where differences in both capture and detection are in concern, it is important to differentiate between the particular strategies that exist specifically for the detection step. However an antigen is captured to the plate (by direct adsorption to the surface or through a pre-coated "capture" antibody, as in a sandwich ELISA), it is the detection step (as either direct or indirect detection) that largely determines the sensitivity of an ELISA.

Four Different ELISA Formats
Key Steps in Different ELISA Types
Indirect Direct Sandwich Competitive
Capture Ab Coating X X X
Antigen Coating X
Blocking
Sample (Antigen) Incubation X X
Primary Ab Incubation
Secondary Ab Incubation X
Substrate Prep
Signal Detection
Data Analysis

Compared to the other ELISA formats, the sandwich ELISA has the following advantages:

 High specificity: the antigen/analyte is specifically captured and detected
 Suitable for complex (or crude/impure) samples: the antigen does not require purification prior to measurement
 Flexibility and high sensitivity: both direct or indirect detection methods can be used

The diagram below shows the schematics for Boster’s sandwich ELISA assay. Check our online catalog of complete, ready-to-use sandwich ELISA Kits below... Choose the kit for your purposes from a selection of almost 1000!

Boster’s sandwich ELISA assay
For more free ELISA technical resources, just click this button…
View ELISA Resources
Top Rated ELISA kits on Biocompare
Human TNF Alpha PicoKine™ ELISA Kit EK0525 Human IL-2 PicoKine™ ELISA Kit EK0397 Human MMP-9 PicoKine™ ELISA Kit EK0465
Human TNF Alpha PicoKine™ ELISA Kit EK0525 Human IL-2 PicoKine™ ELISA Kit EK0397 Human MMP-9 PicoKine™ ELISA Kit EK0465
Human IL-12(p40) PicoKine™ ELISA Kit EK0423 Human IL-6 PicoKine™ ELISA Kit EK0410 Human G-CSF PicoKine™ ELISA Kit EK0360
Human IL-12(p40) PicoKine™ ELISA Kit EK0423 Human IL-6 PicoKine™ ELISA Kit EK0410 Human G-CSF PicoKine™ ELISA Kit EK0360
Shop for Kits!