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	March 20, 2017

Dear Earthling,

Are you preparing for a FACS experiment? Here’s a 10 point checklist to help you prepare for your FACS sorting experiment:

	Perform a cell viability count using the trypan blue exclusion method.
	Remove any existing clumps in the cell suspension by sieving cells through a nylon mesh.
	Adjust the cell concentration to 107-108 per ml.
	Ensure that all staining controls are included in the panel.
	Check that the correct nozzle is installed.
	Check that the sheath fluid levels are adequate.
	Ensure that the collection media had serum and HEPES buffer added to it.
	Check that the collection tubes are protein pre-coated ones.
	Make sure all the lasers and filters are functioning properly.
	Adjust the threshold level of the instrument as required.

Boster Products for Flow Cytometry



Anti-Human/Mouse CD45R (B220) Antibody PE Conjugated, Flow Validated	Anti-Human CD3 Antibody FITC Conjugated, Flow Validated	Anti-Human CD19 Antibody PerCP-Cyanine5.5 Conjugated, Flow Validated



Anti-Human CD3 Antibody APC Conjugated, Flow Validated	Anti-Human CD45 Antibody PerCP Conjugated, Flow Validated	Anti-Human CD71 Antibody Unconjugated, Flow Validated