FACS Sorting Preparation Checklist

Dear Earthling,

Are you preparing for a FACS experiment? Here’s a 10 point checklist to help you prepare for your FACS sorting experiment:

• Perform a cell viability count using the trypan blue exclusion method.
• Remove any existing clumps in the cell suspension by sieving cells through a nylon mesh.
• Adjust the cell concentration to 107-108 per ml.
• Ensure that all staining controls are included in the panel.
• Check that the correct nozzle is installed.
• Check that the sheath fluid levels are adequate.
• Ensure that the collection media had serum and HEPES buffer added to it.
• Check that the collection tubes are protein pre-coated ones.
• Make sure all the lasers and filters are functioning properly.
• Adjust the threshold level of the instrument as required.