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Product Info Summary
|Description:||Transient overexpression lysate of MKI67 (FHA domain) interacting nucleolar phosphoprotein (MKI67IP)|
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NIFK (NM_032390) Human Over-expression Lysate
Transient overexpression lysate of MKI67 (FHA domain) interacting nucleolar phosphoprotein (MKI67IP)
Storage & Handling
Cite This Product
NIFK (NM_032390) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084365)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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NIFK Human Over-expression Lysates NM_032390.
Western blot validation of overexpression lysate (LS084365) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with NIFK (Myc-DDK-tagged) Human nucleolar protein interacting with the FHA domain of MKI67 (NIFK) using transfection reagent.
Gene/Protein Information for NIFK (Source: Uniprot.org, NCBI)
MKI67 FHA domain-interacting nucleolar phosphoprotein
MKI67 FHA domain-interacting nucleolar phosphoprotein NIFK MKI67IP, Nopp34 nucleolar protein interacting with the FHA domain of MKI67 MKI67 FHA domain-interacting nucleolar phosphoprotein|hNIFK|nucleolar phosphoprotein Nopp34|nucleolar protein interacting with the FHA domain of pKi-67*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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