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Product Info Summary
|Description:||Transient overexpression lysate of PITPNM family member 3 (PITPNM3), transcript variant 1|
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NIR1 (PITPNM3) (NM_031220) Human Over-expression Lysate
Transient overexpression lysate of PITPNM family member 3 (PITPNM3), transcript variant 1
Storage & Handling
Cite This Product
NIR1 (PITPNM3) (NM_031220) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS083394)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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NIR1 (PITPNM3) Human Over-expression Lysates NM_031220.
Western blot validation of overexpression lysate (LS083394) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PITPNM3 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for PITPNM3 (Source: Uniprot.org, NCBI)
Membrane-associated phosphatidylinositol transfer protein 3
PtdIns transfer protein family
membrane-associated phosphatidylinositol transfer protein 3; MGC157740; MGC157741; NIR-1; NIR1CORD5; Phosphatidylinositol transfer protein, membrane-associated 3; PITPnm 3; PITPNM family member 3; Pyk2 N-terminal domain-interacting receptor 1; RDGBA3; retinal degeneration B alpha 3 PITPNM3 ACKR6, CORD5, NIR1, RDGBA3 PITPNM family member 3 membrane-associated phosphatidylinositol transfer protein 3|NIR-1|PITPnm 3|PYK2 N-terminal domain-interacting receptor 1|atypical chemokine receptor 6|cone rod dystrophy 5|phosphatidylinositol transfer protein, membrane-associated 3|retinal degeneration B alpha 3*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on PITPNM3, check out the PITPNM3 Infographic
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