nkx6.2 (NKX6-2) (NM_177400) Human Over-expression Lysate

Lysate of NKX6-2 over-expression cell lines, WB control

Transient overexpression lysate of NK6 homeobox 2 (NKX6-2)

Product Info Summary

SKU: LS084504
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of NK6 homeobox 2 (NKX6-2)

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Product Name

nkx6.2 (NKX6-2) (NM_177400) Human Over-expression Lysate

See all NKX6-2 products

SKU/Catalog Number

LS084504

Size

100 µg

Description

Transient overexpression lysate of NK6 homeobox 2 (NKX6-2)

Storage & Handling

Cite This Product

nkx6.2 (NKX6-2) (NM_177400) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084504)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for NKX6-2 (Source: Uniprot.org, NCBI)

Gene Name

NKX6-2

Full Name

Homeobox protein Nkx-6.2

Weight

29.263kDa

Alternative Names

Homeobox protein Nkx-6.2 NKX6-2 GTX, NKX6.2, NKX6B, SPAX8 NK6 homeobox 2 homeobox protein Nkx-6.2|NK homeobox family 6, B|NK6 transcription factor related, locus 2|glial and testis-specific homeobox protein|homeobox 6B|homeobox protein NK-6 homolog B

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on NKX6-2, check out the NKX6-2 Infographic

NKX6-2 infographic

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In this infographic, you will see the following information for NKX6-2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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