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Product Info Summary
|Description:||Transient overexpression lysate of NLR family, CARD domain containing 5 (NLRC5)|
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NLRC5 (NM_032206) Human Over-expression Lysate
Transient overexpression lysate of NLR family, CARD domain containing 5 (NLRC5)
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Cite This Product
NLRC5 (NM_032206) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084166)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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NLRC5 Human Over-expression Lysates NM_032206.
Western blot validation of overexpression lysate (LS084166) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with NLRC5 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for NLRC5 (Source: Uniprot.org, NCBI)
Caterpiller Protein 16.1; CLR16.1; NLR Family CARD Domain Containing 5; NOD27; NOD4; NOD-Like Receptor C5; Nucleotide-Binding Oligomerization Domain Protein 27; Nucleotide-Binding Oligomerization Domain Protein 4; Nucleotide-Binding Oligomerization Domains 27; Protein NLRC5 NLRC5 CLR16.1, NOD27, NOD4 NLR family CARD domain containing 5 protein NLRC5|NOD-like receptor C5|caterpiller protein 16.1|nucleotide-binding oligomerization domain protein 4|nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 5|nucleotide-binding oligomerization domains 27*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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