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Product Info Summary
|Description:||Transient overexpression lysate of N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase) (NPL)|
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NPL (NM_030769) Human Over-expression Lysate
Transient overexpression lysate of N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase) (NPL)
Storage & Handling
Cite This Product
NPL (NM_030769) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS080896)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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NPL Human Over-expression Lysates NM_030769.
Western blot validation of overexpression lysate (LS080896) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with NPL (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for NPL (Source: Uniprot.org, NCBI)
N-acetylneuraminate lyase NPL C112, C1orf13, NAL1, NPL N-acetylneuraminate pyruvate lyase N-acetylneuraminate lyase|N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase)|N-acetylneuraminic acid aldolase|NALase|dihydrodipicolinate synthetase homolog 1|sialate-pyruvate lyase|sialic acid aldolase*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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