OCT2 Colorimetric Cell-Based ELISA Kit

44106 ELISA kit, cell-based

The OCT2 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor OCT2 protein expression profile in cells. The kit can be used for measuring the relative amounts of OCT2 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on OCT2.

Product Info Summary

SKU: EKC1004
Size: 1 kit, containing 1 plates and all necessary reagents
Reactive Species: Human, Mouse
Application: ELISA

Product Name

OCT2 Colorimetric Cell-Based ELISA Kit

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SKU/Catalog Number

EKC1004

Size

1 kit, containing 1 plates and all necessary reagents

*Question: How many samples can I assay/run in this kit?

Description

The OCT2 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor OCT2 protein expression profile in cells. The kit can be used for measuring the relative amounts of OCT2 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on OCT2.

Storage & Handling

4°C/6 Months

Cite This Product

OCT2 Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1004)

Clonality of Antibodies

See Datasheet for details

Sensitivity

>5000 cells

Assay Range

> 5000 Cells

Reactive Species

EKC1004 is reactive to POU2F2 in Human, Mouse samples

Application Guarantee

EKC1004 is guaranteed for ELISA in Human, Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-44106 Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

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Gene/Protein Information For POU2F2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P09086

Gene ID

5452

Gene Name

POU2F2

Full Name

POU domain, class 2, transcription factor 2

Weight

51.209kDa

Superfamily

POU transcription factor family

Alternative Names

homeobox protein; Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; OCT2Oct-2; Octamer-binding protein 2; Octamer-binding transcription factor 2; OTF-2; OTF2POU domain class 2, transcription factor 2; POU class 2 homeobox 2; POU domain, class 2, transcription factor 2 POU2F2 OCT2, OTF2, Oct-2 POU class 2 homeobox 2 POU domain, class 2, transcription factor 2|OTF-2|homeobox protein|lymphoid-restricted immunoglobulin octamer-binding protein NF-A2|octamer-binding protein 2|octamer-binding transcription factor 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on POU2F2, check out the POU2F2 Infographic

POU2F2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for POU2F2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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