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Product Info Summary
|Description:||Transient overexpression lysate of opsin 1 (cone pigments), long-wave-sensitive (OPN1LW)|
OPN1LW (NM_020061) Human Over-expression Lysate
Transient overexpression lysate of opsin 1 (cone pigments), long-wave-sensitive (OPN1LW)
Storage & Handling
Cite This Product
OPN1LW (NM_020061) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005956)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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OPN1LW Human Over-expression Lysates NM_020061.
Western blot validation of overexpression lysate (LS005956) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with OPN1LW (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for OPN1LW (Source: Uniprot.org, NCBI)
Long-wave-sensitive opsin 1
G-protein coupled receptor 1 family
Long-wave-sensitive opsin 1 OPN1LW CBBM, CBP, COD5, RCP, ROP opsin 1, long wave sensitive long-wave-sensitive opsin 1|cone dystrophy 5 (X-linked)|opsin 1 (cone pigments), long-wave-sensitive|red cone opsin|red cone photoreceptor pigment|red-sensitive opsin*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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No publications found for LS005956
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