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Product Info Summary
|Description:||Transient overexpression lysate of olfactory receptor, family 2, subfamily C, member 3 (OR2C3)|
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OR2C3 (NM_198074) Human Over-expression Lysate
Transient overexpression lysate of olfactory receptor, family 2, subfamily C, member 3 (OR2C3)
Storage & Handling
Cite This Product
OR2C3 (NM_198074) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS081472)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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OR2C3 Human Over-expression Lysates NM_198074.
Western blot validation of overexpression lysate (LS081472) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with OR2C3 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for OR2C3 (Source: Uniprot.org, NCBI)
Olfactory receptor 2C3
G-protein coupled receptor 1 family
olfactory receptor 2C3; Olfactory receptor 2C4; Olfactory receptor 2C5; Olfactory receptor OR1-30; olfactory receptor, family 2, subfamily C, member 3; olfactory receptor, family 2, subfamily C, member 4; olfactory receptor, family 2, subfamily C, member 5 pseudogene; OR2C4; OR2C5P; OST742 OR2C3 OR2C4, OR2C5P, OST742 olfactory receptor family 2 subfamily C member 3 olfactory receptor 2C3|olfactory receptor 2C4|olfactory receptor 2C5|olfactory receptor OR1-30|olfactory receptor, family 2, subfamily C, member 4|olfactory receptor, family 2, subfamily C, member 5 pseudogene*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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