Click image to see more details
Product Info Summary
| SKU: | EKC1424 |
|---|---|
| Size: | 1 kit, containing one 96-well plate and all necessary reagents |
| Reactive Species: | Human |
| Application: | ELISA |
Customers Who Bought This Also Bought
Product info
Product Name
p47 phox Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC1424
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The p47 phox Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor p47 phox protein expression profile in cells. The kit can be used for measuring the relative amounts of p47 phox in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on p47 phox.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
p47 phox Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1424)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1424 is reactive to Ncf1 in Human samples
Application Guarantee
EKC1424 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-Ncf1 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 6ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
Submit A Review
Data Examples, Quality Control Data
Click image to see more details
Boster Kit Box
Click image to see more details
Western blot analysis of extracts from HT-29/COLO205/HepG2 cells
Protein Target Info & Infographic
Gene/Protein Information For Ncf1 (Source: Uniprot.Org, NCBI)
Gene Name
Ncf1
Full Name
Neutrophil cytosol factor 1
Weight
44.667kDa
Alternative Names
47 kDa neutrophil oxidase factor; FLJ79451; NADPH oxidase organizer 2; NCF-1; NCF1A; neutrophil cytosol factor 1,47 kDa autosomal chronic granulomatous disease protein; neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal1); neutrophil cytosolic factor 1; neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1); Neutrophil NADPH oxidase factor 1; Nox organizer 2; NOXO2NCF-47K; Nox-organizing protein 2; p47phox; SH3 and PX domain-containing protein 1A; SH3PXD1Ap47-phox Ncf1|NCF-47K, NO, NOXO2, Ncf-, Ncf-1, p47-phox, p47
For more info on Ncf1, check out the Ncf1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Ncf1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For p47 phox Colorimetric Cell-Based ELISA Kit (EKC1424)
Hello CJ!
No publications found for EKC1424
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used p47 phox Colorimetric Cell-Based ELISA Kit?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review p47 phox Colorimetric Cell-Based ELISA Kit
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
