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- Table of Contents
Facts about Neutrophil cytosol factor 1.
Mouse | |
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Gene Name: | Ncf1 |
Uniprot: | Q09014 |
Entrez: | 17969 |
Belongs to: |
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No superfamily |
47 kDa neutrophil oxidase factor; FLJ79451; NADPH oxidase organizer 2; NCF-1; NCF1A; neutrophil cytosol factor 1,47 kDa autosomal chronic granulomatous disease protein; neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal1); neutrophil cytosolic factor 1; neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1); Neutrophil NADPH oxidase factor 1; Nox organizer 2; NOXO2NCF-47K; Nox-organizing protein 2; p47phox; SH3 and PX domain-containing protein 1A; SH3PXD1Ap47-phox
Mass (kDA):
44.667 kDA
Mouse | |
---|---|
Location: | 5 G2|5 74.47 cM |
Sequence: | 5; |
BosterBio experiments can be optimized with flow procedures. But, there are many different options and nuances that must be considered. These tips and tricks will help you optimize the results of your experiments with Boster Bio. To make sure you're using the NCF1 marker efficiently, read on for some history of Steven Boster and his work. After reading the following tips and tricks, you'll be well on your way to optimizing your experiments with Boster Bio.
If you are preparing to optimize your ELISA by using the NCF1 marker, there are several things to consider before you start your experiment. To minimize contamination and maximize detection, the primary antibody should be concentrated. Important is also the blocking buffer. Boster Bio provides optimization tips and guides to assist you in optimizing your experiments. We will answer some of the most common questions you might have in this article.
Steven Boster's 1993 debut product was his first product. That was also when he developed PicoKine(tm), his proprietary ELISA technology. The patent-pending ELISA platform used trade secrets to deliver high sensitivity ELISA kits. Steven Boster's company was the largest Chinese antibody catalog in the 1990s. His name is synonymous to quality, efficiency, as well as reliability.
After a battle with COVID-19 in which he was defeated, Steve Boster, the father of Nina Mae Hall, died in Madison on June 6, 2022. He was the father to Donald, Sr., David Boster, as well as Nina Mae Hall. Frances Boster is his wife of 52years. They have two daughters, Crystal Boster & Natosha Peeck. He also has six grandchildren. There are four brothers, Jack Boster & Sandra Blanton. His sister Lisa Milton is also surviving. Steve Boster is survived in death by numerous nieces or nephews.
Boster Bio's latest boster bioproduct identifies NCF1, an extracellular protein, melanosome fractions and aortic samples. The antibody recognizes the protein by its ability to cross-react and is extremely specific. Because it is specific, it can help researchers determine if their sample is genetically related to another person's.
NCF1 polymorphisms are associated with lower levels in reactive oxygen species (ROS), and inflammatory cells. This association with autoimmunity opens up new perspectives into the pathogenesis and progression of complex autoimmune disorders. The Ncf1 proteins is critical for the catalysis in electron transfer from NADPH and oxygen. It is a key component to the NADPH oxidase compound. ROS is generated during this process. High levels of these radicals can lead to arthritis.
The NCF1 genes are associated with inflammation, autoreactive T cells, and other factors. It can be used as a target to develop immunotherapeutic medications. Studies have shown that mice lacking Ncf1 were more susceptible to zymosan-induced joint disease in mice. These results are based only on an experimental model for arthritis, in which the Ncf1 mutant does not significantly influence the development and progression of the disease.
To perform this experiment, we used platinumTaq DNA Polymerase in GeneAmp 9700. We used the Akita primers, 5'-CCTGGGTTCATGTGGCTGATG-3', and the Ncf1 primers, 5'-GGAGAGAGCCCTCTCA-3', to detect the neomycin gene. To detect the Neomycin gene, we used an additional reverse prime.
This gene may have a greater impact on T cell-dependent, autoimmune disease. In a mouse model, Ncf1 gene was also associated to severe EAE. This study demonstrates a link between Ncf1 gene expression and the Pia4 gene. So, the Ncf1 gene is not specifically associated with EAE, but the expression of NCF1 in the lung was associated with the severity of the disease in this animal model.
The antibody is specifically designed to stain osteopontin-deficient mice. The Ncf1 protein is found in low levels in the mouse. It is expressed by a truncated version of the protein. The antibody is a powerful marker to distinguish between the wild-type and mutations of the splice site. The NCF1 gene has been associated with numerous diseases, including osteoporosis.
PMID: 8119734 by Jackson S.H., et al. Cloning and functional expression of the mouse homologue of p47phox.
PMID: 9490028 by Mizuki K., et al. Functional modules and expression of mouse p40(phox) and p67(phox), SH3-domain-containing proteins involved in the phagocyte NADPH oxidase complex.