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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-NCF1 Antibody Picoband™
Boster Bio Anti-NCF1 Antibody Picoband™ catalog # A01586-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NCF1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01586-1)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E. coli-derived human NCF1 recombinant protein (Position: M1-D270).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A01586-1 is reactive to NCF1 in Human, Mouse, Rat
A01586-1 is guaranteed for ELISA, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NCF1 using anti-NCF1 antibody (A01586-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human blood lysates,
Lane 3: rat thymus tissue lysates,
Lane 4: rat lung tissue lysates,
Lane 5: mouse NIH3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCF1 antigen affinity purified polyclonal antibody (Catalog # A01586-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCF1 at approximately 47KD. The expected band size for NCF1 is at 44KD.
Gene/Protein Information For NCF1 (Source: Uniprot.Org, NCBI)
Neutrophil cytosol factor 1
47 kDa neutrophil oxidase factor; FLJ79451; NADPH oxidase organizer 2; NCF-1; NCF1A; neutrophil cytosol factor 1,47 kDa autosomal chronic granulomatous disease protein; neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal1); neutrophil cytosolic factor 1; neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1); Neutrophil NADPH oxidase factor 1; Nox organizer 2; NOXO2NCF-47K; Nox-organizing protein 2; p47phox; SH3 and PX domain-containing protein 1A; SH3PXD1Ap47-phox NCF1 CGD1A, NOXO2, SH3PXD1A, p47phox, NCF1 neutrophil cytosolic factor 1 neutrophil cytosol factor 1|47 kDa autosomal chronic granulomatous disease protein|47 kDa neutrophil oxidase factor|NADPH oxidase organizer 2|NCF-1|NCF-47K|SH3 and PX domain-containing protein 1A|neutrophil NADPH oxidase factor 1|neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1)|nox organizer 2|nox-organizing protein 2|p47-phox*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on NCF1, check out the NCF1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for NCF1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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