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Product Info Summary
|Description:||Transient overexpression lysate of proteasomal ATPase-associated factor 1 (PAAF1)|
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PAAF1 (NM_025155) Human Over-expression Lysate
Transient overexpression lysate of proteasomal ATPase-associated factor 1 (PAAF1)
Storage & Handling
Cite This Product
PAAF1 (NM_025155) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS080227)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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PAAF1 Human Over-expression Lysates NM_025155.
Western blot validation of overexpression lysate (LS080227) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PAAF1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for PAAF1 (Source: Uniprot.org, NCBI)
Proteasomal ATPase-associated factor 1
WD repeat PAAF1/RPN14 family
FLJ11848; PAAF; proteasomal ATPase-associated factor 1; Protein G-16; Rpn14; WD repeat domain 71; WD repeat-containing protein 71; WDR71 PAAF1 PAAF, Rpn14, WDR71 proteasomal ATPase associated factor 1 proteasomal ATPase-associated factor 1|WD repeat domain 71|WD repeat-containing protein 71*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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