Product Info Summary
SKU: | LS000116 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of adenylate cyclase activating polypeptide 1 (pituitary) (ADCYAP1), transcript variant 1 |
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Product info
Product Name
PACAP (ADCYAP1) (NM_001099733) Human Over-expression Lysate
See all PACAP/ADCYAP1 products
SKU/Catalog Number
LS000116
Size
100 µg
Description
Transient overexpression lysate of adenylate cyclase activating polypeptide 1 (pituitary) (ADCYAP1), transcript variant 1
Storage & Handling
Cite This Product
PACAP (ADCYAP1) (NM_001099733) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS000116)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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PACAP (ADCYAP1) Human Over-expression Lysates NM_001099733.
Western blot validation of overexpression lysate (LS000116) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ADCYAP1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
PACAP (ADCYAP1) Human Over-expression Lysates NM_001099733.
Western blot validation of overexpression lysate (LS000116) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ADCYAP1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
PACAP (ADCYAP1) Human Over-expression Lysates NM_001117.
Western blot validation of overexpression lysate (LS000116) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ADCYAP1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for ADCYAP1 (Source: Uniprot.org, NCBI)
Gene Name
ADCYAP1
Full Name
Pituitary adenylate cyclase-activating polypeptide
Weight
18.835kDa
Superfamily
glucagon family
Alternative Names
ADCYAP1; adenylate cyclase activating polypeptide 1 (pituitary); PACAP; PACAPMGC126852; pituitary adenylate cyclase-activating polypeptide ADCYAP1 PACAP adenylate cyclase activating polypeptide 1 pituitary adenylate cyclase-activating polypeptide|adenylate cyclase activating polypeptide 1 (pituitary)|prepro-PACAP
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on ADCYAP1, check out the ADCYAP1 Infographic
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In this infographic, you will see the following information for ADCYAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PACAP (ADCYAP1) (NM_001099733) Human Over-expression Lysate (LS000116)
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No publications found for LS000116
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