PAMM (FAM213A) (NM_032333) Human Over-expression Lysate

Lysate of FAM213A over-expression cell lines, WB control

Transient overexpression lysate of chromosome 10 open reading frame 58 (C10orf58), transcript variant 1

Product Info Summary

SKU: LS084293
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of chromosome 10 open reading frame 58 (C10orf58), transcript variant 1

Product Name

PAMM (FAM213A) (NM_032333) Human Over-expression Lysate

See all FAM213A products

SKU/Catalog Number

LS084293

Size

100 µg

Description

Transient overexpression lysate of chromosome 10 open reading frame 58 (C10orf58), transcript variant 1

Storage & Handling

Cite This Product

PAMM (FAM213A) (NM_032333) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084293)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for Prxl2a (Source: Uniprot.org, NCBI)

Gene Name

Prxl2a

Full Name

Peroxiredoxin-like 2A

Weight

24.395kDa

Superfamily

peroxiredoxin-like PRXL2 family

Alternative Names

C10orf58; Chromosome 10 Open Reading Frame 58; Family With Sequence Similarity 213, Member A; PAMM; Peroxiredoxin (PRX)-Like 2 Activated In M-CSF Stimulated Monocytes; Peroxiredoxin-Like 2 Activated In M-CSF Stimulated Monocytes; Protein PAMM; Redox-Regulatory Protein PAMM; UPF0765 Protein C10orf58 Prxl2a|5730469M10Rik, AU040822, Ad, Adrx, Fam21, Fam213a, Pamm|peroxiredoxin like 2A|peroxiredoxin-like 2A|Adiporedoxin|UPF0765 protein C10orf58 homolog|family with sequence similarity 213, member A|peroxiredoxin-like 2 activated in M-CSF stimulated monocytes|redox-regulatory protein FAM213A|redox-regulatory protein PAMM

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on Prxl2a, check out the Prxl2a Infographic

Prxl2a infographic

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In this infographic, you will see the following information for Prxl2a: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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