PCDH11X (NM_032968) Human Over-expression Lysate

Lysate of PCDH11X over-expression cell lines, WB control

Transient overexpression lysate of protocadherin 11 X-linked (PCDH11X), transcript variant c

Product Info Summary

SKU: LS027328
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of protocadherin 11 X-linked (PCDH11X), transcript variant c

Product Name

PCDH11X (NM_032968) Human Over-expression Lysate

See all PCDH11X products

SKU/Catalog Number

LS027328

Size

100 µg

Description

Transient overexpression lysate of protocadherin 11 X-linked (PCDH11X), transcript variant c

Storage & Handling

Cite This Product

PCDH11X (NM_032968) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS027328)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for PCDH11X (Source: Uniprot.org, NCBI)

Gene Name

PCDH11X

Full Name

Protocadherin-11 X-linked

Weight

147.558kDa

Alternative Names

KIAA1326; PCDH11protocadherin-S; PCDHXprotocadherin 11X; PCDH-Xprotocadherin-11 X-linked; protocadherin 11 X-linked; protocadherin 11; Protocadherin on the X chromosome; Protocadherin-11; Protocadherin-S PCDH11X PCDH-X, PCDH-Y, PCDH11, PCDH11Y, PCDH22, PCDHX, PPP1R119 protocadherin 11 X-linked protocadherin-11 X-linked|Protocadherin on the Y chromosome|Protocadherin prostate cancer|Protocadherin-11 Y-linked|Protocadherin-22|Protocadherin-PC|protein phosphatase 1, regulatory subunit 119|protocadherin 11X|protocadherin on the X chromosome|protocadherin-S

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on PCDH11X, check out the PCDH11X Infographic

PCDH11X infographic

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In this infographic, you will see the following information for PCDH11X: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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