PCTAIRE2 (CDK17) (NM_002595) Human Over-expression Lysate

Lysate of PCTAIRE2 over-expression cell lines, WB control

Transient overexpression lysate of cyclin-dependent kinase 17 (CDK17), transcript variant 1

Product Info Summary

SKU: LS005128
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of cyclin-dependent kinase 17 (CDK17), transcript variant 1

Product Name

PCTAIRE2 (CDK17) (NM_002595) Human Over-expression Lysate

See all PCTAIRE2 products

SKU/Catalog Number

LS005128

Size

100 µg

Description

Transient overexpression lysate of cyclin-dependent kinase 17 (CDK17), transcript variant 1

Storage & Handling

Cite This Product

PCTAIRE2 (CDK17) (NM_002595) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005128)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for CDK17 (Source: Uniprot.org, NCBI)

Gene Name

CDK17

Full Name

Cyclin-dependent kinase 17

Weight

59.582kDa

Superfamily

protein kinase superfamily

Alternative Names

Cell division protein kinase 17; cyclin-dependent kinase 17; EC 2.7.11; EC 2.7.11.22; PCTAIRE protein kinase 2; PCTAIRE2; PCTAIRE-motif protein kinase 2; PCTK2; protein kinase cdc2-related PCTAIRE-2; Serine/threonine-protein kinase PCTAIRE-2 CDK17 PCTAIRE2, PCTK2 cyclin dependent kinase 17 cyclin-dependent kinase 17|PCTAIRE-motif protein kinase 2|cell division protein kinase 17|protein kinase cdc2-related PCTAIRE-2|serine/threonine-protein kinase PCTAIRE-2

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on CDK17, check out the CDK17 Infographic

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In this infographic, you will see the following information for CDK17: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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