PDE4D Colorimetric Cell-Based ELISA Kit

Phosphodiesterase 4D ELISA kit, cell-based

The PDE4D Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PDE4D protein expression profile in cells. The kit can be used for measuring the relative amounts of PDE4D in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PDE4D.

Product Info Summary

SKU: EKC1446
Size: 1 kit, containing 1 plates and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA

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Product Name

PDE4D Colorimetric Cell-Based ELISA Kit

See all Phosphodiesterase 4D products

SKU/Catalog Number

EKC1446

Size

1 kit, containing 1 plates and all necessary reagents

*Question: How many samples can I assay/run in this kit?

Description

The PDE4D Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PDE4D protein expression profile in cells. The kit can be used for measuring the relative amounts of PDE4D in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PDE4D.

Storage & Handling

4°C/6 Months

Cite This Product

PDE4D Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1446)

Clonality of Antibodies

See Datasheet for details

Sensitivity

>5000 cells

Assay Range

> 5000 Cells

Reactive Species

EKC1446 is reactive to PDE4D in Human, Mouse, Rat samples

Application Guarantee

EKC1446 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-Phosphodiesterase 4D Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

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Gene/Protein Information For PDE4D (Source: Uniprot.Org, NCBI)

Uniprot ID

Q08499

Gene ID

5144

Gene Name

PDE4D

Full Name

cAMP-specific 3',5'-cyclic phosphodiesterase 4D

Weight

91.115kDa

Superfamily

cyclic nucleotide phosphodiesterase family

Alternative Names

cAMP-specific (dunce (Drosophila)-homologphosphodiesterase E3); cAMP-specific 3'-5'-cyclic phosphodiesterase 4D; cAMP-specific phosphodiesterase PDE4D6; DKFZp686M11213; Drosophila); dunce-like phosphodiesterase E3; EC 3.1.4; FLJ97311; HSPDE4D; phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog; phosphodiesterase 4D, cAMP-specific PDE4D ACRDYS2, DPDE3, HSPDE4D, PDE43N2, STRK1, PDE4D phosphodiesterase 4D cAMP-specific 3,5-cyclic phosphodiesterase 4D|cAMP-specific phosphodiesterase PDE4D6|phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila)|testicular tissue protein Li 136

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PDE4D, check out the PDE4D Infographic

PDE4D infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PDE4D: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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