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Product Info Summary
| SKU: | BA1083 |
|---|---|
| Size: | 0.5ml |
| Application: | ELISA, IHC-P, IHC-F, WB |
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Product info
| Product Name | HRP Conjugated Protein G |
| SKU/Catalog Number | BA1083 |
| Pack Size | 0.5ml |
| Form | Concentrated, Liquid |
| Concentration | 1mg/mL |
| Description | Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria much like Protein A but with differing binding specificities. It is a 65-kDa (G148 protein G) and a 58 kDa (C40 protein G) cell surface protein that has found application in purifying antibodies through its binding to the Fab and Fc region. The native molecule also binds albumin, but because serum albumin is a major contaminant of antibody sources, the albumin binding site has been removed from recombinant forms of Protein G. |
| Contents | 0.5 mg of HRP conjugated protein G 0.01 M PBS (pH7.4) 50% glycerol |
| Labeling Method | Protein G is conjugated to peroxidase by means of a method described by Wilson MB and Nakane PK. (Reference: Wilson MB and Nakane PK. In Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, P215 (1978).) |
| Storage & Expiration | At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. |
| Applications | WB, IHC-P, IHC-F, ELISA |
| Cite This Product | Peroxidase Conjugated Protein G (Boster Biological Technology, Pleasanton CA, USA, Catalog # BA1083) |
Product Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western Blotting: 0.1-0.3μg/ml (ECL detection) Western Blotting: 0.3-2μg/ml (DAB detection) Immunohistochemistry (Paraffin-embedded Section): 5-10μg/ml Immunohistochemistry (Frozen Section): 2-10μg/ml ELISA: 0.025-0.05μg/ml
Validation Images & Assay Conditions
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Boster Kit Box
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