PHCA Colorimetric Cell-Based ELISA Kit

PHCA ELISA kit, cell-based

The PHCA Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PHCA protein expression profile in cells. The kit can be used for measuring the relative amounts of PHCA in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PHCA.

Product Info Summary

SKU: EKC1455
Size: 1 kit, containing one 96-well plate and all necessary reagents
Reactive Species: Human, Mouse
Application: ELISA

Product Name

PHCA Colorimetric Cell-Based ELISA Kit

See all PHCA products

SKU/Catalog Number

EKC1455

Size

1 kit, containing one 96-well plate and all necessary reagents

Description

The PHCA Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PHCA protein expression profile in cells. The kit can be used for measuring the relative amounts of PHCA in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PHCA.

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

PHCA Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1455)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC1455 is reactive to ACER3 in Human, Mouse samples

Application Guarantee

EKC1455 is guaranteed for ELISA in Human, Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-PHCA Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

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Gene/Protein Information For ACER3 (Source: Uniprot.Org, NCBI)

Gene Name

ACER3

Full Name

Alkaline ceramidase 3

Weight

31.552kDa

Superfamily

alkaline ceramidase family

Alternative Names

Alkaline CDase 3; alkaline ceramidase 3; Alkaline dihydroceramidase SB89; Alkaline phytoceramidase; aPHC; APHCAlkCDase 3; EC 3.5.1.-; FLJ11238; PHCA; phytoceramidase, alkaline ACER3 APHC, PHCA, PLDECO alkaline ceramidase 3 alkaline ceramidase 3|alkCDase 3|alkaline CDase 3|alkaline dihydroceramidase SB89|alkaline phytoceramidase|phytoceramidase, alkaline

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ACER3, check out the ACER3 Infographic

ACER3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ACER3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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