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Product Info Summary
|Description:||Transient overexpression lysate of phospholipase A2, group XIIA (PLA2G12A)|
PLA2G12A (NM_030821) Human Over-expression Lysate
Transient overexpression lysate of phospholipase A2, group XIIA (PLA2G12A)
Storage & Handling
Cite This Product
PLA2G12A (NM_030821) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS081579)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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PLA2G12A Human Over-expression Lysates NM_030821.
Western blot validation of overexpression lysate (LS081579) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PLA2G12A (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for PLA2G12A (Source: Uniprot.org, NCBI)
Group XIIA secretory phospholipase A2
phospholipase A2 family
EC 126.96.36.199; group XII secreted phospholipase A2; group XIIA secreted phospholipase A2; group XIIA secretory phospholipase A2; GXII sPLA2; GXII; Phosphatidylcholine 2-acylhydrolase 12A; phospholipase A2, group XII; phospholipase A2, group XIIA; PLA2G12; ROSSY; sPLA2-XII*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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No publications found for LS081579
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