PLAT (NM_000930) Human Over-expression Lysate

Lysate of t-Plasminogen Activator/tPA over-expression cell lines, WB control

Transient overexpression lysate of plasminogen activator, tissue (PLAT), transcript variant 1

Product Info Summary

SKU: LS005327
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of plasminogen activator, tissue (PLAT), transcript variant 1

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Product Name

PLAT (NM_000930) Human Over-expression Lysate

See all t-Plasminogen Activator/tPA products

SKU/Catalog Number

LS005327

Size

100 µg

Description

Transient overexpression lysate of plasminogen activator, tissue (PLAT), transcript variant 1

Storage & Handling

Cite This Product

PLAT (NM_000930) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005327)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for PLAT (Source: Uniprot.org, NCBI)

Uniprot ID

P00750

Gene ID

5327

Gene Name

PLAT

Full Name

Tissue-type plasminogen activator

Weight

62.917kDa

Superfamily

peptidase S1 family

Alternative Names

Alteplase; DKFZp686I03148; EC 3.4.21; EC 3.4.21.68; plasminogen activator, tissue type; plasminogen activator, tissue; PLAT; Reteplase; tissue plasminogen activator (t-PA); tissue-type plasminogen activator; TPA; T-PA; tPlasminogen Activator; t-Plasminogen Activator PLAT T-PA, TPA plasminogen activator, tissue type tissue-type plasminogen activator|alteplase|plasminogen/activator kringle|reteplase|t-plasminogen activator

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on PLAT, check out the PLAT Infographic

PLAT infographic

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In this infographic, you will see the following information for PLAT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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