PLEKHO2 (NM_025201) Human Over-expression Lysate

Lysate of PLEKHO2 over-expression cell lines, WB control

Transient overexpression lysate of pleckstrin homology domain containing, family O member 2 (PLEKHO2)

Product Info Summary

SKU: LS080301
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of pleckstrin homology domain containing, family O member 2 (PLEKHO2)

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Product Name

PLEKHO2 (NM_025201) Human Over-expression Lysate

See all PLEKHO2 products

SKU/Catalog Number

LS080301

Size

100 µg

Description

Transient overexpression lysate of pleckstrin homology domain containing, family O member 2 (PLEKHO2)

Storage & Handling

Cite This Product

PLEKHO2 (NM_025201) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS080301)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for PLEKHO2 (Source: Uniprot.org, NCBI)

Uniprot ID

Q8TD55

Gene ID

80301

Gene Name

PLEKHO2

Full Name

Pleckstrin homology domain-containing family O member 2

Weight

53.35kDa

Alternative Names

DKFZp761K2312; FLJ38884; PH domain-containing family O member 2; PH domain-containing family Q member 1; PH domain-containing protein; pleckstrin homology domain containing, family O member 2; pleckstrin homology domain containing, family Q member 1; pleckstrin homology domain-containing family O member 2; Pleckstrin homology domain-containing family Q member 1; PLEKHQ1; PP1628; pp9099 PLEKHO2 PLEKHQ1, PP1628, pp9099 pleckstrin homology domain containing O2 pleckstrin homology domain-containing family O member 2|PH domain-containing family O member 2|PH domain-containing family Q member 1|PH domain-containing protein|pleckstrin homology domain-containing family Q member 1

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on PLEKHO2, check out the PLEKHO2 Infographic

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