Click image to see more details
Product Info Summary
|Description:||Transient overexpression lysate of polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 1|
Customers Who Bought This Also Bought
POLR1B (NM_019014) Human Over-expression Lysate
Transient overexpression lysate of polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 1
Storage & Handling
Cite This Product
POLR1B (NM_019014) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084172)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
Click image to see more details
POLR1B Human Over-expression Lysates NM_019014.
Western blot validation of overexpression lysate (LS084172) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with POLR1B (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for POLR1B (Source: Uniprot.org, NCBI)
DNA-directed RNA polymerase I subunit RPA2
RNA polymerase beta chain family
DNA-directed RNA polymerase I 135 kDa polypeptide; DNA-directed RNA polymerase I 135kDa polypeptide; DNA-directed RNA polymerase I subunit RPA2; EC 220.127.116.11; FLJ10816; FLJ21921; MGC131780; polymerase (RNA) I polypeptide B, 128kDa; RNA polymerase I subunit 2; RPA116; RPA135; RPA2; Rpo1-2 Polr1b|128kD, 128kDa, D630020H17Rik, R, RPA, RPA116, RPA135, RPA2, Rpo, Rpo1-2|polymerase (RNA) I polypeptide B|DNA-directed RNA polymerase I subunit RPA2|DNA-directed RNA polymerase I 135 kDa polypeptide|DNA-directed RNA polymerase I 135kDa polypeptide|RNA polymerase 1-2, 128 kDa subunit|RNA polymerase I subunit 2*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on POLR1B, check out the POLR1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POLR1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for LS084172
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Here are featured tools and databases that you might find useful:
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Have you used POLR1B (NM_019014) Human Over-expression Lysate?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review POLR1B (NM_019014) Human Over-expression Lysate
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question