Anti-Fruit fly CG6353 Antibody

Boster Bio Anti-CG6353 Antibody catalog # DZ33921-1. This antibody reacts with Fruit fly. Independently reviewed in 1 review(s).

Product Info Summary

SKU: DZ33921-1
Size: 200 μl/vial
Reactive Species: Fruit fly
Host: Rabbit
Application: IHC-P

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Product Name

Anti-Fruit fly CG6353 Antibody

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SKU/Catalog Number

DZ33921-1

Size

200 μl/vial

Form

Liquid

Description

Boster Bio Anti-CG6353 Antibody catalog # DZ33921-1. This antibody reacts with Fruit fly.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Fruit fly CG6353 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ33921-1)

Host

Rabbit

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

Clonality

Polyclonal

Clone Number

23N41

Isotype

Rabbit IgG

Immunogen

E.coli-derived human RBM45 recombinant protein (Position: A2-R215+Q370-Y476).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

DZ33921-1 is reactive to in Fruit fly

Applications

DZ33921-1 is guaranteed for IHC-P Boster Guarantee

Observed Molecular Weight

16 kDa

Calculated molecular weight

46663 MW

Background of

Recombination activating gene 1 also known as RAG-1 is a protein that in humans is encoded by the RAG1 gene. The protein encoded by this gene is involved in activation of immunoglobulin V-D-J recombination. The encoded protein is involved in recognition of the DNA substrate, but stable binding and cleavage activity also requires RAG2. Defects in this gene can be the cause of several diseases.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry(Paraffin-embedded Section), 1μg/ml, Hu, Ms

Validation Images & Assay Conditions

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1 Reviews For Anti-Fruit fly CG6353 Antibody

0

Immunofluorescence for Anti-CG6353 Antibody--Amin Ghabrial  Pathology and Cell Biology  Columbia University  Principal Investigator

Excellent

             
                No Image Available              

"My lab is characterizing a mutant we named disjointed. The disjointed gene is also known as CG6353 or Archease. We have used this antibody for IF, and it appears to work well. It gives us a staining pattern for the endogenous protein that matches the pattern we see with an HA-tagged fusion protein detected by anti-HA staining."

                             
Application Immunofluorescence
Sample 5% BSA as a blocking agent for 30 min at 37°C
Sample Drosophila larvae
Primary Incubation  1:100 at 4 degrees overnight
Blocking Agent 4% horse serum
Secondary Incubation   1:1000 anti-Rb-A488
Tertiary Incubation  N/A
Detection IF
Results Summary Antibody staining reveals subcellular localization of the protein. In salivary gland and trachea, localization was primarily nuclear.
                             
                         
       

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Order DetailsPrice
DZ33921-1

200μl

$470
DZ33921-1-Biotin

200 μl Biotin conjugated

$670
DZ33921-1-Cy3

200 μl Cy3 conjugated

$670
DZ33921-1-Dylight488

200 μl Dylight488 conjugated

$670
DZ33921-1-Dylight550

200 μl Dylight550 conjugated

$670
DZ33921-1-Dylight594

200 μl Dylight594 conjugated

$670
DZ33921-1-FITC

200 μl FITC conjugated

$670
DZ33921-1-HRP

200 μl HRP conjugated

$670
DZ33921-1-APC

200 μl APC conjugated

$770
DZ33921-1-PE

200 μl PE conjugated

$770
DZ33921-1-carrier-free

Carrier Free

$470

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Eddy test
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DZ33921-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00

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