Click image to see more details
Product Info Summary
| SKU: | DZ41152 |
|---|---|
| Size: | 200 μl/vial |
| Reactive Species: | Fruit fly |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Fruit fly emp Antibody
SKU/Catalog Number
DZ41152
Size
200 μl/vial
Form
Liquid
Description
Boster Bio Polyclonal Anti-Fruit fly emp Antibody catalog # DZ41152. This antibody reacts with Fruit fly.
Storage & Handling
At -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.
Cite This Product
Anti-Fruit fly emp Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ41152)
Host
Rabbit
Contents
Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Reactive Species
DZ41152 is reactive to emp in Fruit fly
Application & Images
Applications
DZ41152 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:1,000
Positive Control
WB: normal drosophila melanogaster head tissues, CG2727 KO / +, normal drosophila melanogaster thorax tissues, CG2727 KO / +
Validation Images & Assay Conditions
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Western blot analysis of Emp using anti-Emp antibody (DZ41152).
Electrophoresis was performed on a 7.5% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: normal drosophila melanogaster head tissues,
Lane 2: CG2727 KO / +,
Lane 3: normal drosophila melanogaster thorax tissues,
Lane 4: CG2727 KO / +.
After electrophoresis, proteins were transferred to PVDF at 20 V overnight. Blocked the membrane with 5% skim milk in PBS-T for 30 min at RT. The membrane was incubated with rabbit anti-Emp antigen affinity purified polyclonal antibody (Catalog # DZ41152) at 1:1000 dilution in PBS-T with 1% skim milk for 1h at RT, then washed with PBS 3 times and probed with a goat anti-rabbit IgG-HRP secondary antibody at 1:10000 dilution PBS-T with 1% skim milk for 1h at RT, then washed with PBS 3 times. The signal is detected with Immobilon Forte Western HRP substrate. A specific band was detected for Emp at approximately 59 kDa.
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Customer Reviews
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1 Reviews For Anti-Fruit fly emp Antibody
Western blot for Polyclonal Anti-Drosophila melanogaster (Fruit fly) emp Antibody--Yuzuru Imai, Department of Research for Parkinson's Disease Juntendo University Graduate School of Medicine
Excellent
| SKU | DZ41152 |
|---|---|
| Application | Western blot |
| Sample | Drosophila melanogaster (head and thorax) |
| Detection | Immobilon Forte Western HRP substrate |
"There are non-specific bands and immunostaining may not be possible, but Western blotting is sufficient with this antibody."
Yuzuru Imai
Verified customer
Submitted 2021-04-29
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