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emp antibody (DZ41152) performs well in WB and shows reactivity with Fruit fly.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Western blot analysis of Emp using anti-Emp antibody (DZ41152).
Electrophoresis was performed on a 7.5% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: normal drosophila melanogaster head tissues,
Lane 2: CG2727 KO / +,
Lane 3: normal drosophila melanogaster thorax tissues,
Lane 4: CG2727 KO / +.
After electrophoresis, proteins were transferred to PVDF at 20 V overnight. Blocked the membrane with 5% skim milk in PBS-T for 30 min at RT. The membrane was incubated with rabbit anti-Emp antigen affinity purified polyclonal antibody (Catalog # DZ41152) at 1:1000 dilution in PBS-T with 1% skim milk for 1h at RT, then washed with PBS 3 times and probed with a goat anti-rabbit IgG-HRP secondary antibody at 1:10000 dilution PBS-T with 1% skim milk for 1h at RT, then washed with PBS 3 times. The signal is detected with Immobilon Forte Western HRP substrate. A specific band was detected for Emp at approximately 59 kDa.
Electrophoresis was performed on a 7.5% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: normal drosophila melanogaster head tissues,
Lane 2: CG2727 KO / +,
Lane 3: normal drosophila melanogaster thorax tissues,
Lane 4: CG2727 KO / +.
After electrophoresis, proteins were transferred to PVDF at 20 V overnight. Blocked the membrane with 5% skim milk in PBS-T for 30 min at RT. The membrane was incubated with rabbit anti-Emp antigen affinity purified polyclonal antibody (Catalog # DZ41152) at 1:1000 dilution in PBS-T with 1% skim milk for 1h at RT, then washed with PBS 3 times and probed with a goat anti-rabbit IgG-HRP secondary antibody at 1:10000 dilution PBS-T with 1% skim milk for 1h at RT, then washed with PBS 3 times. The signal is detected with Immobilon Forte Western HRP substrate. A specific band was detected for Emp at approximately 59 kDa.
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Anti-Fruit fly emp Antibody
emp antibody
| Reactive Species | Host | Application |
|---|---|---|
| Fruit fly | Rabbit | WB |
Tested Applications
emp antibody was positively detected by WB in:
normal drosophila melanogaster head tissues, CG2727 KO / +, normal drosophila melanogaster thorax tissues, CG2727 KO / +
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
1:1000
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Fruit fly emp Antibody
View all EMP Antibodies
View all EMP Antibodies
Form
Liquid
Uniprot
Storage & Handling
At -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Cite This Product
Anti-Fruit fly emp Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ41152)
Protocols
Western Blot protocol for emp antibody
Review
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1 Review For Anti-Fruit fly emp Antibody
Western blot for Polyclonal Anti-Drosophila melanogaster (Fruit fly) emp Antibody--Yuzuru Imai, Department of Research for Parkinson's Disease Juntendo University Graduate School of Medicine
| SKU | DZ41152 |
|---|---|
| Application | Western blot |
| Sample | Drosophila melanogaster (head and thorax) |
| Detection | Immobilon Forte Western HRP substrate |
"There are non-specific bands and immunostaining may not be possible, but Western blotting is sufficient with this antibody."
Yuzuru Imai
Verified customer
Submitted 2021-04-29
Product Promise
We promise Anti-Fruit fly emp Antibody performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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DZ41152
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026