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Product Info Summary
| SKU: | DZ41142 |
|---|---|
| Size: | 200 μl/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
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Product info
Product Name
Anti-Human METTL23 Antibody
SKU/Catalog Number
DZ41142
Size
200 μl/vial
Form
Liquid
Description
Boster Bio Polyclonal Anti-Human METTL23 Antibody catalog # DZ41142. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human METTL23 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ41142)
Host
Rabbit
Contents
Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Reactive Species
DZ41142 is reactive to METTL23 in Human, Mouse
Calculated molecular weight
21.5 kDa
Application & Images
Positive Control
WB: human HCT whole cell, human Caco-2 whole cell, human SHY whole cell, human U87 whole cell, mouse GL261 whole cell
Validation Images & Assay Conditions
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Western blot analysis of METTL23 using anti-METTL23 antibody (DZ41142).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human HCT whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human SHY whole cell lysates,
Lane 4: human U87 whole cell lysates,
Lane 5: mouse GL261 whole cell lysates.
After Electrophoresis, proteins were transferred to a PVDF membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-METTL23 antigen affinity purified polyclonal antibody (Catalog # DZ41142) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and detected by Licor IR800 rabbit antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
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Customer Reviews
Have you used Anti-Human METTL23 Antibody?
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1 Reviews For Anti-Human METTL23 Antibody
Mettl23 Detection in Cell Lines
Excellent
Source: Biocompare.com
| SKU | DZ41142 |
|---|---|
| Application | Western Blot |
| Sample | Human cell lines |
| Primary Incubation | 1:500 with 1% BSA in TBS, incubated overnight, at 4oC, with shaking |
| Blocking Agent | 5% BSA in TBS |
| Secondary Incubation | Licor IRDye 800 Goat anti Rabbit diluted 1:10000 with 1% BSA in TBS, incubated for 1 hour at RT , with shaking |
| Detection | Licor Odyssey IR Scanner |
| Additional Notes | The antibody has very good stability at room temperature (transit took over 2 weeks and the ice packs had long melted before delivery). |
| Results Summary | The antibody gave a detectable band at the expected size for all cell lines tested with no non-specific bands. |
"This antibody was used to determine the level of protein expression in various cell lines in order to set up two groups (high vs low expression). This antibody was custom made because numerous suppliers have either stopped their production for this antibody or quality/specificity was low."
Byron Baron
Verified customer
Submitted 2024-07-16
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