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METTL23 antibody (DZ41142) and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Western blot analysis of METTL23 using anti-METTL23 antibody (DZ41142).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human HCT whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human SHY whole cell lysates,
Lane 4: human U87 whole cell lysates,
Lane 5: mouse GL261 whole cell lysates.
After Electrophoresis, proteins were transferred to a PVDF membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-METTL23 antigen affinity purified polyclonal antibody (Catalog # DZ41142) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and detected by Licor IR800 rabbit antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human HCT whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human SHY whole cell lysates,
Lane 4: human U87 whole cell lysates,
Lane 5: mouse GL261 whole cell lysates.
After Electrophoresis, proteins were transferred to a PVDF membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-METTL23 antigen affinity purified polyclonal antibody (Catalog # DZ41142) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and detected by Licor IR800 rabbit antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
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Anti-Human METTL23 Antibody
METTL23 antibody
| Reactive Species | Host |
|---|---|
| Human, Mouse | Rabbit |
Tested Applications
METTL23 antibody was positively detected by WB in:
human HCT whole cell, human Caco-2 whole cell, human SHY whole cell, human U87 whole cell, mouse GL261 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Product Information
Product Name
Anti-Human METTL23 Antibody
View all METTL23 Antibodies
View all METTL23 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Calculated Molecular Weight
21.5 kDa
Cite This Product
Anti-Human METTL23 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ41142)
Protocols
Western Blot protocol for METTL23 antibody
Review
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1 Review For Anti-Human METTL23 Antibody
Mettl23 Detection in Cell Lines
Source: Biocompare.com
| SKU | DZ41142 |
|---|---|
| Application | Western Blot |
| Sample | Human cell lines |
| Primary Incubation | 1:500 with 1% BSA in TBS, incubated overnight, at 4oC, with shaking |
| Blocking Agent | 5% BSA in TBS |
| Secondary Incubation | Licor IRDye 800 Goat anti Rabbit diluted 1:10000 with 1% BSA in TBS, incubated for 1 hour at RT , with shaking |
| Detection | Licor Odyssey IR Scanner |
| Additional Notes | The antibody has very good stability at room temperature (transit took over 2 weeks and the ice packs had long melted before delivery). |
| Results Summary | The antibody gave a detectable band at the expected size for all cell lines tested with no non-specific bands. |
"This antibody was used to determine the level of protein expression in various cell lines in order to set up two groups (high vs low expression). This antibody was custom made because numerous suppliers have either stopped their production for this antibody or quality/specificity was low."
Byron Baron
Verified customer
Submitted 2024-07-16
Product Promise
We promise Anti-Human METTL23 Antibody performs excellently in relevant applications . If it doesn't perform as promised, we will refund the product. Learn more
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DZ41142
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026