Click image to see more details
Product Info Summary
| SKU: | DZ33938-1 |
|---|---|
| Size: | 200 μl/vial |
| Reactive Species: | Fruit fly |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Fruit fly Me31B Antibody
SKU/Catalog Number
DZ33938-1
Size
200 μl/vial
Form
Liquid
Description
Boster Bio Polyclonal Anti-Me31B Antibody catalog # DZ33938-1. This antibody reacts with Fruit fly.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fruit fly Me31B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ33938-1)
Host
Rabbit
Contents
Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Reactive Species
DZ33938-1 is reactive to me31B in Fruit fly
Application & Images
Applications
DZ33938-1 is guaranteed for WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 1:10 | 000 |
Validation Images & Assay Conditions
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Western blot analysis of Me31B using anti-Fruit fly Me31B antibody (DZ33938-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with non-fat dry milk for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fruit fly Me31B antibody (DZ33938-1) at 1:10,000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10,000, incubation 1h at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Me31B at approximately 52 kDa. The expected band size for Me31B is at 52 kDa.
Specific Publications For Anti-Fruit fly Me31B Antibody (DZ33938-1)
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Customer Reviews
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1 Reviews For Anti-Fruit fly Me31B Antibody
A Good Drosophila Me31B Antibody for WB--Svetlana Dzitoyeva, University of Colorado, Denver, Biochemistry and Molecular Genetics, Research Scientist
Excellent
Source: Biocompare.com
| SKU | DZ33938-1 |
|---|---|
| Application | Western Blot |
| Sample | Insect, D. melanogaster |
| Detection | ECL |
"The research of our lab aims to understand the mechanisms of maternally deposited RNAs and proteins clearance during maternal-to-zygotic transition (MZT). This is a very good AB, tested on WB only. The image shows GFP-tagged Me31B. Very good and clean. Clean and sharp band. Recommend Boster antibody production service."
Svetlana Dzitoyeva
Verified customer
Submitted 2020-01-10
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