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Product Info Summary
| SKU: | DZ11111-1 |
|---|---|
| Size: | 200 μl/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC-P |
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Product info
Product Name
Anti-Human RBM45 Antibody
SKU/Catalog Number
DZ11111-1
Size
200 μl/vial
Form
Liquid
Description
Boster Bio Polyclonal Anti-RBM45 Antibody catalog # DZ11111-1. Tested in IHC-P application. This antibody reacts with human and mouse.
Storage & Handling
At -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.
Cite This Product
Anti-Human RBM45 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ11111-1)
Host
Rabbit
Contents
Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN3.
Clonality
Polyclonal
Clone Number
23N41
Isotype
Rabbit IgG
Immunogen
E.coli-derived human RBM45 recombinant protein (Position: A2-R215+Q370-Y476).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
DZ11111-1 is reactive to ABCC12 in Human, Mouse
Applications
DZ11111-1 is guaranteed for IHC-P Boster Guarantee
Observed Molecular Weight
16 kDa
Calculated molecular weight
152.297kDa
Background of ABCC12
Recombination activating gene 1 also known as RAG-1 is a protein that in humans is encoded by the RAG1 gene. The protein encoded by this gene is involved in activation of immunoglobulin V-D-J recombination. The encoded protein is involved in recognition of the DNA substrate, but stable binding and cleavage activity also requires RAG2. Defects in this gene can be the cause of several diseases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 1μg/ml, Hu, Ms
Validation Images & Assay Conditions
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Figure 1. IHC analysis of RBM45 using anti-RBM45 antibody (DZ11111-1).
RBM45 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- RBM45 (DZ11111-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 2. IHC analysis of RBM45 using anti-RBM45 antibody (DZ11111-1).
RBM45 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- RBM45 (DZ11111-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For ABCC12 (Source: Uniprot.org, NCBI)
Gene Name
ABCC12
Full Name
Multidrug resistance-associated protein 9
Weight
152.297kDa
Superfamily
ABC transporter superfamily
Alternative Names
ATP-binding cassette transporter sub-family C member 12; ATP-binding cassette, sub-family C (CFTR/MRP), member 12; MGC27071; MRP9ATP-binding cassette sub-family C member 12; multidrug resistance-associated protein 9 ABCC12 MRP9 ATP binding cassette subfamily C member 12 ATP-binding cassette sub-family C member 12|ATP-binding cassette transporter sub-family C member 12|ATP-binding cassette, sub-family C (CFTR/MRP), member 12|multidrug resistance-associated protein 9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ABCC12, check out the ABCC12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ABCC12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human RBM45 Antibody (DZ11111-1)
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