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Product Info Summary
| SKU: | DZ11111-1 |
|---|---|
| Size: | 200 μl/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC-P |
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Product info
Product Name
Anti-Human RBM45 Antibody
SKU/Catalog Number
DZ11111-1
Size
200 μl/vial
Form
Liquid
Description
Boster Bio Polyclonal Anti-RBM45 Antibody catalog # DZ11111-1. Tested in IHC-P application. This antibody reacts with human and mouse.
Storage & Handling
At -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.
Cite This Product
Anti-Human RBM45 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ11111-1)
Host
Rabbit
Contents
Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human RBM45 recombinant protein (Position: A2-R215+Q370-Y476).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
DZ11111-1 is reactive to in Human, Mouse
Reconstitution
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
DZ11111-1 is guaranteed for IHC-P Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 1μg/ml, Hu, Ms
Positive Control
IHC: human mammary cancer tissue, mouse testis tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of RBM45 using anti-RBM45 antibody (DZ11111-1).
RBM45 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- RBM45 (DZ11111-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 2. IHC analysis of RBM45 using anti-RBM45 antibody (DZ11111-1).
RBM45 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- RBM45 (DZ11111-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-Human RBM45 Antibody (DZ11111-1)
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