Anti-Zebrafish Ush2a Antibody

IF analysis of Ush2a using anti-Ush2a antibody (DZ01481).
Ush2a was detected in cryosections of wildtype zebrafish retina tissue without prior fixation. The tissue section was blocked with Blocking buffer (10% NGS, 2% BSA, 0.1% Tween in PBS) for 30 minutes. The tissue section was then incubated with rabbit anti-Ush2a Antibody (DZ01481) in blocking solution at 1:200 dilution overnight at 4°C. Alexa Fluor® 568 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody a and incubated for 1 hour at room temperature in blocking solution. Post immuno-fixation with 4% PFA in PBS for 10 minutes. Mount in Prolong gold.

Analysis of usherin and Whrnb expression in retinal sections of wild-type, adgrv1rmc22, and adgrv1Δexon40-42 zebrafish.
Retinal cryosections of wild-type, mutant adgrv1rmc22, and adgrv1Δexon40-42 zebrafish larvae (5 dpf) labeled with antibodies directed against Whrnb (red) (A) or usherin (red) (B) and centrin (green). Nuclei are counterstained with DAPI (blue). (A) Both in wild-type and in adgrv1Δexon40-42 zebrafish larvae, Whrnb was present at the photoreceptor periciliary region in close proximity to centrin. Quantification revealed that the Whrnb signal intensity in adgrv1Δexon40-42 retinal sections is similar to that in wild-type sections, whereas a significant reduction of Whrnb signal was observed in retinae of adgrv1rmc22 larvae. Magnified images are shown at the right side. Bar graphs represent the mean fluorescence intensity of anti-Whrnb staining of all photoreceptors of a single, central section of one larval zebrafish eye (n = 14 eyes). (B) Both in wild-type and adgrv1Δexon40-42 zebrafish larvae, usherin was present at the photoreceptor periciliary region in close proximity to centrin. Quantification reveals that the usherin signal intensity in retinal sections of adgrv1Δexon40-42 larvae is similar to that in sections of wild types, whereas a significant reduction of usherin signal was observed in retinae of adgrv1rmc22 larvae. Bar graphs represent the mean fluorescence intensity of anti-usherin staining of all photoreceptors of a single, central section of one larval zebrafish eye, with mean ± SD (n = 14 eyes). Data were analyzed using one-way ANOVA followed by Tukey’s multiple comparison test (∗∗p < 0.01; ∗∗∗∗p < 0.0001). Scale bars, 10 μm.
Index in PubMed under a CC BY license. PMID: 41036464