Product Info Summary
SKU: | LS085313 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of peptidylprolyl isomerase (cyclophilin)-like 4 (PPIL4) |
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Product info
Product Name
PPIL4 (NM_139126) Human Over-expression Lysate
SKU/Catalog Number
LS085313
Size
100 µg
Description
Transient overexpression lysate of peptidylprolyl isomerase (cyclophilin)-like 4 (PPIL4)
Storage & Handling
Cite This Product
PPIL4 (NM_139126) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS085313)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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Protein Target Info & Infographic
Gene/Protein Information for Ppil4 (Source: Uniprot.org, NCBI)
Gene Name
Ppil4
Full Name
Peptidyl-prolyl cis-trans isomerase-like 4
Weight
57.23kDa
Superfamily
cyclophilin-type PPIase family
Alternative Names
Cyclophilin-like protein PPIL4; cyclophilin-type peptidyl-prolyl cis-trans isomerase; EC 5.2.1.8; HDCME13P; peptidyl-prolyl cis-trans isomerase-like 4; peptidylprolyl isomerase (cyclophilin)-like 4; PPIase; Rotamase PPIL4; serologically defined breast cancer antigen NY-BR-18
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on Ppil4, check out the Ppil4 Infographic
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In this infographic, you will see the following information for Ppil4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PPIL4 (NM_139126) Human Over-expression Lysate (LS085313)
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