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Product Info Summary
| SKU: | EK2376 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Primate |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma(heparin, EDTA) |
Product info
Product Name
Primate TNF-alpha ELISA Kit PicoKine®
SKU/Catalog Number
EK2376
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Primate TNF-alpha ELISA Kit PicoKine® (96 Tests). Quantitate Primate TNF-alpha in cell culture supernatants, serum, plasma (heparin, EDTA). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Primate TNF-alpha ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2376)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: Val77-Leu233
Capture/Detection Antibodies
polyclonal antibody from goat|polyclonal antibody from goat
Sensitivity
<5 pg/mL
Assay Range
31.3-2,000 pg/mL
Standard Dilution Instructions
See datasheet of EK2376 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2376 is reactive to TNF in Primate samples
Validated Sample Types
cell culture supernatants, serum, plasma(heparin, EDTA)
Application Guarantee
EK2376 is guaranteed for ELISA in Primate by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TNF
TNFα (Tumor Necrosis Factor alpha) gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2376-CAP | Anti-Primate TNF Pre-coated 96-well strip microplate | 1 |
| EK2376-ST | Primate TNF Standard | 2 vials, 10 ng/tube |
| EK2376-DA | Primate TNF Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.3 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.044 | 0.089 | 0.151 | 0.253 | 0.451 | 0.772 | 1.28 | 1.9 |
Data Example Images
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Primate TNF-alpha PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Primate TNF-alpha ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 56 | 239 | 544 | 60 | 125 | 888 |
| Standard deviation | 4.00 | 11.07 | 32.86 | 3.79 | 7.20 | 39.69 |
| CV (%) | 7.2% | 4.6% | 6.0% | 6.3% | 5.8% | 4.5% |
Specific Publications For Primate TNF-alpha ELISA Kit PicoKine® (EK2376)
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