Product Info Summary
SKU: | LS005612 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of protein-kinase, interferon-inducible double stranded RNA dependent inhibitor, repressor of (P58 repressor) (PRKRIR) |
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Product info
Product Name
PRKRIR (THAP12) (NM_004705) Human Over-expression Lysate
SKU/Catalog Number
LS005612
Size
100 µg
Description
Transient overexpression lysate of protein-kinase, interferon-inducible double stranded RNA dependent inhibitor, repressor of (P58 repressor) (PRKRIR)
Storage & Handling
Cite This Product
PRKRIR (THAP12) (NM_004705) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005612)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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PRKRIR (THAP12) Human Over-expression Lysates NM_004705.
Western blot validation of overexpression lysate (LS005612) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with THAP12 (Myc-DDK-tagged) Human THAP domain containing 12 (THAP12)
Protein Target Info & Infographic
Gene/Protein Information for THAP12 (Source: Uniprot.org, NCBI)
Gene Name
THAP12
Full Name
52 kDa repressor of the inhibitor of the protein kinase
Weight
87.704kDa
Alternative Names
52 kDa repressor of the inhibitor of the protein kinase THAP12 DAP4, P52rIPK, PRKRIR, THAP0 THAP domain containing 12 52 kDa repressor of the inhibitor of the protein kinase|58 kDa interferon-induced protein kinase-interacting protein|P58IPK-regulatory protein|THAP domain-containing protein 0|THAP domain-containing protein 12|death-associated protein 4|inhibitor of protein kinase PKR|p58IPK-interacting protein|protein-kinase, interferon-inducible double stranded RNA dependent inhibitor, repressor of (P58 repressor)|testicular tissue protein Li 133
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on THAP12, check out the THAP12 Infographic
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In this infographic, you will see the following information for THAP12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PRKRIR (THAP12) (NM_004705) Human Over-expression Lysate (LS005612)
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No publications found for LS005612
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