PRKRIR (THAP12) (NM_004705) Human Over-expression Lysate

Lysate of THAP12 over-expression cell lines, WB control

Transient overexpression lysate of protein-kinase, interferon-inducible double stranded RNA dependent inhibitor, repressor of (P58 repressor) (PRKRIR)

Product Info Summary

SKU: LS005612
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of protein-kinase, interferon-inducible double stranded RNA dependent inhibitor, repressor of (P58 repressor) (PRKRIR)

Product Name

PRKRIR (THAP12) (NM_004705) Human Over-expression Lysate

See all THAP12 products

SKU/Catalog Number

LS005612

Size

100 µg

Description

Transient overexpression lysate of protein-kinase, interferon-inducible double stranded RNA dependent inhibitor, repressor of (P58 repressor) (PRKRIR)

Storage & Handling

Cite This Product

PRKRIR (THAP12) (NM_004705) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005612)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for THAP12 (Source: Uniprot.org, NCBI)

Gene Name

THAP12

Full Name

52 kDa repressor of the inhibitor of the protein kinase

Weight

87.704kDa

Alternative Names

52 kDa repressor of the inhibitor of the protein kinase THAP12 DAP4, P52rIPK, PRKRIR, THAP0 THAP domain containing 12 52 kDa repressor of the inhibitor of the protein kinase|58 kDa interferon-induced protein kinase-interacting protein|P58IPK-regulatory protein|THAP domain-containing protein 0|THAP domain-containing protein 12|death-associated protein 4|inhibitor of protein kinase PKR|p58IPK-interacting protein|protein-kinase, interferon-inducible double stranded RNA dependent inhibitor, repressor of (P58 repressor)|testicular tissue protein Li 133

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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