Product Info Summary
SKU: | LS005713 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (PSMD7) |
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Product info
Product Name
PSMD7 (NM_002811) Human Over-expression Lysate
SKU/Catalog Number
LS005713
Size
100 µg
Description
Transient overexpression lysate of proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (PSMD7)
Storage & Handling
Cite This Product
PSMD7 (NM_002811) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005713)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
Click image to see more details
PSMD7 Human Over-expression Lysates NM_002811.
Western blot validation of overexpression lysate (LS005713) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PSMD7 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for PSMD7 (Source: Uniprot.org, NCBI)
Gene Name
PSMD7
Full Name
26S proteasome non-ATPase regulatory subunit 7
Weight
37.025kDa
Superfamily
peptidase M67A family
Alternative Names
26S proteasome non-ATPase regulatory subunit 7; 26S proteasome regulatory subunit RPN8; Moloney leukemia virus-34 proviral integration; Mov34 homolog; Mov34 protein homolog; MOV3426S proteasome regulatory subunit S12; MOV34L; P40; proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog); proteasome (prosome, macropain) 26S subunit, non-ATPase, 7; Proteasome subunit p40; Rpn8; S12 PSMD7 MOV34, P40, Rpn8, S12 proteasome 26S subunit, non-ATPase 7 26S proteasome non-ATPase regulatory subunit 7|26S proteasome regulatory subunit S12|26S proteasome regulatory subunit rpn8|Moloney leukemia virus-34 proviral integration|Mov34 homolog|proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog)|proteasome subunit p40
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on PSMD7, check out the PSMD7 Infographic
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In this infographic, you will see the following information for PSMD7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PSMD7 (NM_002811) Human Over-expression Lysate (LS005713)
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