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Product Info Summary
|Description:||Transient overexpression lysate of RAB1A, member RAS oncogene family (RAB1A), transcript variant 1|
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RAB1A (NM_004161) Human Over-expression Lysate
Transient overexpression lysate of RAB1A, member RAS oncogene family (RAB1A), transcript variant 1
Storage & Handling
Cite This Product
RAB1A (NM_004161) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005861)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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RAB1A Human Over-expression Lysates NM_004161.
Western blot validation of overexpression lysate (LS005861) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RAB1A (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for RAB1A (Source: Uniprot.org, NCBI)
Ras-related protein Rab-1A
small GTPase superfamily
DKFZp564B163; member RAS oncogene family; RAB1A, member RAS oncogene family; ras-related protein Rab-1A RAB1A RAB1, YPT1 RAB1A, member RAS oncogene family ras-related protein Rab-1A|GTP binding protein Rab1a|RAB1, member RAS oncogene family|Rab GTPase YPT1 homolog|YPT1-related protein*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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No publications found for LS005861
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