Rat BMP-2 ELISA Kit PicoKine™

Rat BMP-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Bmp2 in bone tissue, cell culture supernatants and serum. Sensitivity: 2pg/ml. Cited in 2 publication(s).

Product Info Summary

SKU: EK0312
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: bone tissue, cell culture supernatants and serum.

Product Name

Rat BMP-2 ELISA Kit PicoKine™

See all Bmp2 products

SKU/Catalog Number

EK0312

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat BMP-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Bmp2 in bone tissue, cell culture supernatants and serum. Sensitivity: 2pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Rat BMP-2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0312)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q283-R396

Sensitivity

<2 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0312 is reactive to Bmp2 in Rat samples

Validated Sample Types

bone tissue, cell culture supernatants and serum.

Application Guarantee

EK0312 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0312-CAP Anti-Rat Bmp2 Pre-coated 96-well strip microplate 1
EK0312-ST Rat Bmp2 Standard 2 vials, 10 ng/tube
EK0312-DA Rat Bmp2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0020.0260.0600.1140.3490.6081.1521.989

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Bmp2, Dilution ratio of 1:1.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat BMP-2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)96245806105234777
Standard deviation6.7211.2749.169.2414.9752.83
CV (%)7%4.6%6.1%8.8%6.4%6.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Bmp2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 19696106100994.094.1%
Sample 224525824222524211.754.8%
Sample 380690291883686546.095.3%
*number of samples for each test n=16.

Gene/Protein Information For Bmp2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P49001

Gene ID

29373

Gene Name

Bmp2

Full Name

Bone morphogenetic protein 2

Weight

44.383kDa

Superfamily

TGF-beta family

Alternative Names

BDA2; BMP2; BMP-2; BMP-2A; BMP2ABone morphogenetic protein 2A; bone morphogenetic protein 2; SSFSC Bmp2|bone morphogenetic protein 2|bone morphogenetic protein 2|BMP-2|BMP-2A|bone morphogenetic protein 2A

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Bmp2, check out the Bmp2 Infographic

Bmp2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Bmp2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0312 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Sevoflurane-induced memory impairment in the postnatal developing mouse brain

Thermally induced self-agglomeration 3D scaffolds with BMP-2-loaded core?shell fibers for enhanced osteogenic differentiation of rat adipose-derived stem cells

Have you used Rat BMP-2 ELISA Kit PicoKine™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Rat BMP-2 ELISA Kit PicoKine™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

3 Customer Q&As for Rat BMP-2 ELISA Kit PicoKine™

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

N. Walker

Verified customer

Asked: 2019-10-11

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-10-11

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2019-07-25

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-07-25

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2019-02-11

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-02-11

$400

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
EK0312
$400.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.