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Product Info Summary
| SKU: | EK1889 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Rat CPE/Carboxypeptidase E ELISA Kit PicoKine®
View all Carboxypeptidase E/CPE ELISA kits
SKU/Catalog Number
EK1889
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat CPE ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Cpe in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat CPE/Carboxypeptidase E ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1889)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: L43-F476
Sensitivity
<50 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1889 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1889 is reactive to CPE in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1889 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Carboxypeptidase E/CPE
CPE may play a role in post-translational processing of peptide hormones including preproopiomelanocortin. It is mapped to 16p13.This gene encodes a member of the M14 family of metallocarboxypeptidases. The encoded preproprotein is proteolytically processed to generate the mature peptidase. This peripheral membrane protein cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. This protein may also function independently of its peptidase activity, as a neurotrophic factor that promotes neuronal survival, and as a sorting receptor that binds to regulated secretory pathway proteins, including prohormones. Mutations in this gene are implicated in type 2 diabetes.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1889-CAP | Anti-Rat CPE Pre-coated 96-well strip microplate | 1 |
| EK1889-ST | Rat CPE Standard | 2 vials, 10 ng/tube |
| EK1889-DA | Rat CPE Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.013 | 0.068 | 0.119 | 0.202 | 0.409 | 0.787 | 1.459 | 2.189 |
Data Example Images
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Rat CPE PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Carboxypeptidase E/CPE, Dilution ratio of 1:1, concentration in serum and plasma is 800-1000 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat CPE/Carboxypeptidase E ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 72 | 271 | 858 | 81 | 282 | 926 |
| Standard deviation | 3.96 | 20.87 | 39.47 | 4.70 | 17.48 | 63.89 |
| CV (%) | 5.5% | 7.7% | 4.6% | 5.8% | 6.2% | 6.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Carboxypeptidase E/CPE in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 72 | 65 | 65 | 65 | 66 | 3.03 | 4.5% |
| Sample 2 | 271 | 294 | 289 | 283 | 284 | 8.58 | 3.0% |
| Sample 3 | 858 | 870 | 828 | 820 | 844 | 20.63 | 2.4% |
Protein Target Info & Infographic
Gene/Protein Information For CPE (Source: Uniprot.Org, NCBI)
Gene Name
CPE
Full Name
Carboxypeptidase E
Weight
53.151kDa
Superfamily
peptidase M14 family
Alternative Names
Carboxypeptidase E; Carboxypeptidase H; CPE; CPH; CPH-1; EC 3.4.17; EC 3.4.17.10; Enkephalin convertase; Prohormone-processing carboxypeptidase CPE CPH carboxypeptidase E carboxypeptidase E|carbocypeptidase E|carboxypeptidase H|cobalt-stimulated chromaffin granule carboxypeptidase|enkephalin convertase|epididymis secretory sperm binding protein|insulin granule-associated carboxypeptidase|prohormone-processing carboxypeptidase
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CPE, check out the CPE Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CPE: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat CPE/Carboxypeptidase E ELISA Kit PicoKine® (EK1889)
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No publications found for EK1889
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Rat CPE/Carboxypeptidase E ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-06-14
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-06-14
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-07-21
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-07-21
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Carboxypeptidase E in Rat serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-03-29
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Carboxypeptidase E ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-03-29
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-10-18
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-10-18
Question
Q: if the enzyme conjugated Carboxypeptidase E antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Carboxypeptidase E antigen?
L. Edwards
Verified customer
Asked: 2017-11-01
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2017-11-01
Question
Q: how to analyze ELISA data? I have performed Carboxypeptidase E level in serum.
C. Jones
Verified customer
Asked: 2017-04-14
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2017-04-14
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Carboxypeptidase E using the EK1889 Rat Carboxypeptidase E Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2016-02-26
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2016-02-26
