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Product Info Summary
| SKU: | A01407 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-CPE/Carboxypeptidase E Antibody
SKU/Catalog Number
A01407
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CPE/Carboxypeptidase E Antibody catalog # A01407. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CPE/Carboxypeptidase E Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01407)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the C-terminal region of human CPE. AA range:427-476
Reactive Species
A01407 is reactive to CPE in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
53.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01407 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC: 1:100-300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot (WB) analysis of Mouse Brain cells using CPE Polyclonal antibody.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Rat Brain, antibody was diluted at 1:100.
Specific Publications For Anti-CPE/Carboxypeptidase E Antibody (A01407)
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Customer Q&As
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6 Customer Q&As for Anti-CPE/Carboxypeptidase E Antibody
Question
Would A01407 anti-CPE/Carboxypeptidase E antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
It shows on the product datasheet, A01407 anti-CPE/Carboxypeptidase E antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-19
Question
Can you help my question with product A01407, anti-CPE/Carboxypeptidase E antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Jackson
Verified customer
Asked: 2019-09-06
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01407 anti-CPE/Carboxypeptidase E antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-06
Question
I see that the anti-CPE/Carboxypeptidase E antibody A01407 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-01-03
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-01-03
Question
I was wanting to use your anti-CPE/Carboxypeptidase E antibody for IHC for human amygdala teratocarcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human amygdala teratocarcinoma identification?
W. Krishna
Verified customer
Asked: 2017-12-21
Answer
It shows on the product datasheet, A01407 anti-CPE/Carboxypeptidase E antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human amygdala teratocarcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-21
Question
Will anti-CPE/Carboxypeptidase E antibody A01407 work on pig IHC with brain?
Z. Anderson
Verified customer
Asked: 2015-06-10
Answer
Our lab technicians have not validated anti-CPE/Carboxypeptidase E antibody A01407 on pig. You can run a BLAST between pig and the immunogen sequence of anti-CPE/Carboxypeptidase E antibody A01407 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig brain in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-06-10
Question
Is a blocking peptide available for product anti-CPE/Carboxypeptidase E antibody (A01407)?
T. Evans
Verified customer
Asked: 2013-08-15
Answer
We do provide the blocking peptide for product anti-CPE/Carboxypeptidase E antibody (A01407). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2013-08-15
