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Product Info Summary
| SKU: | EK1883 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Rat Plexin B2/PLXNB2 ELISA Kit PicoKine®
SKU/Catalog Number
EK1883
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat Plexin B2 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Plxnb2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 0.1ng/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat Plexin B2/PLXNB2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1883)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: CHO; Immunogen sequence: L20-P1202
Sensitivity
<50 pg/ml
Assay Range
0.78 ng/ml - 50 ng/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1883 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1883 is reactive to PLXNB2 in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1883 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Plexin B2
Plexin-B2 is a protein that in humans is encoded by the PLXNB2 gene. It is mapped to 7q34. Members of the B class of plexins, such as PLXNB2 are transmembrane receptors that participate in axon guidance and cell migration in response to semaphorins.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1883-CAP | Anti-Rat PLXNB2 Pre-coated 96-well strip microplate | 1 |
| EK1883-ST | Rat PLXNB2 Standard | 2 vials, 50 ng/tube |
| EK1883-DA | Rat PLXNB2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.126 | 0.183 | 0.235 | 0.344 | 0.536 | 0.917 | 1.513 | 2.230 |
Data Example Images
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Rat Plexin B2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Plexin B2, Dilution ratio of 1:5, concentration in serum and plasma is 40-53 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat Plexin B2/PLXNB2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 31 | 190 | 449 | 33 | 201 | 456 |
| Standard deviation | 1.89 | 10.26 | 19.76 | 1.55 | 14.67 | 35.11 |
| CV (%) | 6.1% | 5.4% | 4.4% | 4.7% | 7.3% | 7.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Plexin B2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 31 | 33 | 35 | 36 | 33 | 1.92 | 5.8% |
| Sample 2 | 190 | 161 | 186 | 179 | 179 | 11.11 | 6.2% |
| Sample 3 | 449 | 432 | 432 | 456 | 442 | 10.54 | 2.3% |
Protein Target Info & Infographic
Gene/Protein Information For PLXNB2 (Source: Uniprot.Org, NCBI)
Gene Name
PLXNB2
Full Name
Plexin-B2
Weight
205.127kDa
Superfamily
plexin family
Alternative Names
MM1; MM1KIAA0315dJ402G11.3; Nbla00445; PLEXB2; Plexin B2; plexin-B2; PLXNB2 PLXNB2 MM1, Nbla00445, PLEXB2, dJ402G11.3 plexin B2 plexin-B2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PLXNB2, check out the PLXNB2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PLXNB2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat Plexin B2/PLXNB2 ELISA Kit PicoKine® (EK1883)
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No publications found for EK1883
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Recommended Resources
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Customer Reviews
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Customer Q&As
Have a question?
Find answers in Q&As, reviews.
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10 Customer Q&As for Rat Plexin B2/PLXNB2 ELISA Kit PicoKine®
Question
Q: which procedure should I follow in order to thaw whole blood sample for Plexin B2 ELISA after freezing?
H. Rodriguez
Verified customer
Asked: 2020-10-20
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Plexin B2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-10-20
Question
Q: can I use citrate plasma as samples in Rat Plexin B2 Picokine® ELISA Kit (Catalog # EK1883)?
Verified Customer
Verified customer
Asked: 2020-09-23
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Plexin B2 causing degradation of its protein structure. Plexin B2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Plexin B2 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-09-23
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of Plexin B2 in Rat serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Plexin B2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-08-13
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-07-30
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
H. Collins
Verified customer
Asked: 2018-12-10
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-12-10
Question
Q: how do I analyze ELISA data? I have performed Plexin B2 level in serum.
Verified Customer
Verified customer
Asked: 2018-11-17
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-11-17
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Plexin B2 using the EK1883 Rat Plexin B2 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-03-09
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2018-03-09
Question
Q: if the enzyme conjugated Plexin B2 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Plexin B2 antigen?
S. Taylor
Verified customer
Asked: 2018-01-07
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-01-07
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
H. Smith
Verified customer
Asked: 2016-01-25
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2016-01-25
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
L. Jones
Verified customer
Asked: 2015-07-23
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2015-07-23
