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Product Info Summary
| SKU: | EK0508 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (EDTA). |
Product info
Product Name
Rat SCF/KITLG/Kit ligand ELISA Kit PicoKine®
SKU/Catalog Number
EK0508
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat SCF ELISA Kit PicoKine® (96 Tests). Quantitate Rat Kitlg in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat SCF/KITLG/Kit ligand ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0508)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: Q26-A190
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0508 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0508 is reactive to Kitlg in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (EDTA).
Application Guarantee
EK0508 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Kitlg
Stem Cell Factor (also known as SCF, kit-ligand, KL, or steel factor) is a cytokine that binds to the c-Kit receptor (CD117). It is mapped to 12q21.32. SCF was primarily expressed by perivascular cells throughout the bone marrow, and it plays a role in the regulation of HSCs in the stem cell niche in the bone marrow. SCF can exist both as a transmembrane protein and a soluble protein. This gene plays an important role in the hematopoiesis during embryonic development. During development, the presence of the SCF also plays an important role in the localization of melanocytes, cells that produce melanin and control pigmentation. In addition to it, SCF can promote mast cell adhesion, migration, proliferation, and survival. It also promotes the release of histamine and tryptase, which are involved in the allergic response.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0508-CAP | Anti-Rat Kitlg Pre-coated 96-well strip microplate | 1 |
| EK0508-ST | Rat Kitlg Standard | 2 vials, 10 ng/tube |
| EK0508-DA | Rat Kitlg Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.031 | 0.068 | 0.113 | 0.183 | 0.335 | 0.725 | 1.398 | 2.63 |
Data Example Images
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Rat SCF PicoKine ELISA Kit Standard Curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat SCF/KITLG/Kit ligand ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 61 | 246 | 929 | 60 | 244 | 1009 |
| Standard deviation | 2.56 | 13.77 | 62.24 | 3.24 | 17.32 | 70.63 |
| CV (%) | 4.2% | 5.6% | 6.7% | 5.4% | 7.1% | 7% |
Specific Publications For Rat SCF/KITLG/Kit ligand ELISA Kit PicoKine® (EK0508)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Rat SCF/KITLG/Kit ligand ELISA Kit PicoKine®
Question
Q: how can I thaw whole blood sample for SCF ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-09-28
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test SCF for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-09-28
Question
Q: how to proceed with the analysis of ELISA data? I measured SCF level in plasma.
M. Jackson
Verified customer
Asked: 2020-09-11
Answer
A: please read this article on ELISA data analysis. bosterbio.com/elisa-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/elisa-data-analysis-online
Boster Scientific Support
Answered: 2020-09-11
Question
Q: can I use heparin plasma as samples in Rat SCF Picokine® ELISA Kit (Catalog # EK0508)?
S. Anand
Verified customer
Asked: 2020-01-01
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of SCF causing disruption of its protein structure. SCF may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the SCF ELISA, treating samples with various anticoagulants and decided that EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-01-01
Question
Q: What is the optimal O.D. value for SCF ELISA kit? I performed your SCF ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain SCF even though the O.D. values are not very high?
C. Perez
Verified customer
Asked: 2018-12-18
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this SCF ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of SCF that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-12-18
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
C. Hill
Verified customer
Asked: 2018-03-14
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-03-14
