Rat SCF/KITLG/Kit ligand ELISA Kit PicoKine™

Rat SCF ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Kitlg in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 10pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK0508
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (EDTA).

Product Name

Rat SCF/KITLG/Kit ligand ELISA Kit PicoKine™

See all SCF/c-kit Ligand products

SKU/Catalog Number

EK0508

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat SCF ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Kitlg in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Rat SCF/KITLG/Kit ligand ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0508)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q26-A190

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0508 is reactive to Kitlg in Rat samples

Validated Sample Types

cell culture supernatants, serum and plasma (EDTA).

Application Guarantee

EK0508 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0508-CAP Anti-Rat Kitlg Pre-coated 96-well strip microplate 1
EK0508-ST Rat Kitlg Standard 2 vials, 10 ng/tube
EK0508-DA Rat Kitlg Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)02000200020002000200020002000
O.D.0.0500.1270.1830.3160.5280.9011.6392.445

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat SCF/KITLG/Kit ligand ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)61246929602441009
Standard deviation2.5613.7762.243.2417.3270.63
CV (%)4.2%5.6%6.7%5.4%7.1%7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of SCF/c-kit Ligand in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 161596255592.684.5%
Sample 22462352432482434.942%
Sample 392991790482989438.974.3%
*number of samples for each test n=16.

Gene/Protein Information For Kitlg (Source: Uniprot.Org, NCBI)

Uniprot ID

P21581

Gene ID

60427

Gene Name

Kitlg

Full Name

Kit ligand

Weight

30.712kDa

Superfamily

SCF family

Alternative Names

c-kit Ligand; DCUA; DFNA69; DKFZp686F2250; familial progressive hyperpigmentation 2; FPH2; FPHH; KIT ligand; Kitl; KITLG; KL-1; Mast cell growth factor; MGF; MGFSHEP7; SCF; SCFStem cell factor; SFc-Kit ligand; SHEP7; SLF; steel factor Kitlg|Kitl, Mgf, SCF|KIT ligand|kit ligand|C-kit ligand|hematopoietic growth factor KL|mast cell growth factor|steel factor/kit ligand|stem cell factor KL-1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Kitlg, check out the Kitlg Infographic

Kitlg infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Kitlg: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0508 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

GM-CSF Inhibits c-Kit and SCF Expression by Bone Marrow-Derived Dendritic Cells

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5 Customer Q&As for Rat SCF/KITLG/Kit ligand ELISA Kit PicoKine™

Question

Q: how can I thaw whole blood sample for SCF ELISA after freezing?

Verified Customer

Verified customer

Asked: 2020-09-28

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test SCF for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2020-09-28

Question

Q: how to proceed with the analysis of ELISA data? I measured SCF level in plasma.

M. Jackson

Verified customer

Asked: 2020-09-11

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-09-11

Question

Q: can I use heparin plasma as samples in Rat SCF Picokine® ELISA Kit (Catalog # EK0508)?

S. Anand

Verified customer

Asked: 2020-01-01

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of SCF causing disruption of its protein structure. SCF may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the SCF ELISA, treating samples with various anticoagulants and decided that EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-01-01

Question

Q: What is the optimal O.D. value for SCF ELISA kit? I performed your SCF ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain SCF even though the O.D. values are not very high?

C. Perez

Verified customer

Asked: 2018-12-18

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this SCF ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of SCF that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2018-12-18

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

C. Hill

Verified customer

Asked: 2018-03-14

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-03-14

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