Rat sE-Selectin PicoKine™ Fast ELISA Kit


SKU FEK0936
Size 96wells/kit, with removable strips.
Reactivity Rat
Sample Type cell culture supernates, serum and plasma(heparin, citrate).
Sample Volume 100ul per well
Sensitivity <10pg/ml
Assay Range 156pg/ml-10,000pg/ml

Introduction

Boster's Picokine™ Fast ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Overview

Product Name Rat sE-Selectin PicoKine™ Fast ELISA Kit
SKU/Catalog Number FEK0936
Storage & Handling Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Size 96wells/kit, with removable strips.
Description The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Rat E Selectin/SELE with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. E Selectin/SELE tissue specificity:
Cite This Product Rat sE-Selectin PicoKine™ Fast ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0936)
Sample Type cell culture supernates, serum and plasma(heparin, citrate). Anticoagulant(s): heparin or citrate
*The recommended anticoagulants are proven to not block the antibody binding sites on the target antigen. Please do not collect blood sample with other anticoagulants that are not specified above or contact us to check for feasibility.
Sensitivity <10pg/ml
Assay Range 156pg/ml-10,000pg/ml
Immunogen Expression system for standard: NSO; Immunogen sequence: W22-P494
Reactivity Rat
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Antibody Clonalities Capture Antibody | Detection Antibody:
monoclonal antibody from mouse|polyclonal antibody from goat

Assay Details

Kit Components

Catalog number Description Quantity
FEK0936-CAP 96-well plate precoated with anti-Rat Sele antibody 1
FEK0936-ST lyophilized recombinant Rat Sele standard 10ng/tube
FEK0936-DA biotinylated anti-Rat Sele antibody 130ul
AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul
AR1106-1 sample diluent buffer 30ml
AR1106-2 antibody diluent buffer 12ml
AR1106-3 ABC diluent buffer 12ml
AR1104 TMB color developing agent 10ml
AR1105 TMB stop solution 10ml
PLA-SEA Adhesive cover 4

*Why there is no wash buffer? Our Avidin-Biotin-Peroxidase Diluent contains the detergent (TWEEN) normally present in other companies' ELISA kits. This saves you the step of having to wash with the special wash buffer and achieve similar or better signal to noise ratio. The wash can use regular wash buffers (PBS, TBS etc.) commonly found in labs.

Materials Required But Not Provided

  • Microplate reader in standard size.
  • Automated plate washer.
  • Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • Clean tubes and Eppendorf tubes.
  • Washing buffer (neutral PBS or TBS).
  • Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
  • Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

Typical Data Obtained from Rat sE-Selectin PicoKine™ Fast ELISA Kit

(TMB reaction incubate at 37°C for 25-30min)

Concentration(pg/ml)015631262512502500500010000
O.D.0.0070.1530.2750.5140.8601.2241.6071.831

Intra/Inter Assay Precision

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)3451737442831516214077
Standard deviation17.68132.01292.2414.8149.13309.85
CV(%)4.4%7.6%6.6%4.7%9.2%7.6%

Reproducibility

Three samples with differing target protein concentrations were assayed using four different lots to measure the CV% lot to lot variance.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

LotsLot1 (pg/ml)Lot2 (pg/ml)Lot3 (pg/ml)Lot4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 134537535032134719.175.5%
Sample 217371912207619521919121.356.3%
Sample 344283889405542584157203.634.8%
*number of samples for each test n=16.

*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P98105
Gene Name Sele
Protein Name E-selectin
Alternative Names E-selectin;CD62 antigen-like family member E;Endothelial leukocyte adhesion molecule 1;ELAM-1;Leukocyte-endothelial cell adhesion molecule 2;LECAM2;CD62E;Sele;Elam-1;
Subcellular Localization Cell membrane ; Single-pass type I membrane protein.
Molecular Weight 60080 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis (By similarity). .
Research Areas Rat

*You can search these to find other products in these research areas.
Background E-selectin, also called endothelial-leukocyte adhesion molecule-1(ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils.1 An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions.2 E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells(HPCs) into bone marrow(BM).3 A structurally and functionally related group of genes, lymph node homing receptor(LHR), granule membrane protein 140(GMP-140), and endothelial leukocyte adhesion molecule 1(ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1.4

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FEK0936
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