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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (EDTA, citrate) and urine.|
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Rat Serpin D1 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Rat Serpin D1 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Serpind1 in cell culture supernatants, serum and plasma (EDTA, citrate) and urine. Sensitivity: 0.1ng/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat Serpin D1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1970)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: E24-S479
0.78 ng/ml - 50 ng/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1970 is reactive to Serpind1 in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (EDTA, citrate) and urine.
EK1970 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Serpin D1/Heparin Cofactor II
Heparin cofactor II (HCII), a protein encoded by the SERPIND1 gene, is a coagulation factor that inhibits IIa, and is a cofactor for heparin and dermatan sulfate. It is mapped to 11q23. This gene belongs to the serpin gene superfamily. Serpins play roles in many processes including inflammation, blood clotting, and cancer metastasis. Members of this family have highly conserved secondary structures with a reactive center loop that interacts with the protease active site to inhibit protease activity. This gene encodes a plasma serine protease that functions as a thrombin and chymotrypsin inhibitor. The protein is activated by heparin, dermatan sulfate, and glycosaminoglycans. Allelic variations in this gene are associated with heparin cofactor II deficiency.
|EK1970-CAP||Anti-Rat Serpind1 Pre-coated 96-well strip microplate||1|
|EK1970-ST||Rat Serpind1 Standard||2 vials, 50 ng/tube|
|EK1970-DA||Rat Serpind1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Rat Serpin D1 PicoKine ELISA Kitstandard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Serpin D1/Heparin Cofactor II, Dilution ratio of 1:200, concentration in serum is around 1.2ug/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat Serpin D1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Serpin D1/Heparin Cofactor II in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Serpind1 (Source: Uniprot.Org, NCBI)
Heparin cofactor 2
HC2; HC2LS2; HCF2; HCF2clade D (heparin cofactor), member 1; HC-II; Heparin Cofactor II; HLS2HCII; leuserpin 2; LS2; Protease inhibitor leuserpin-2; Serpin D1; serpin peptidase inhibitor, clade D (heparin cofactor), member 1 Serpind1|rls2var1|serpin family D member 1|heparin cofactor 2|HC-II|heparin cofactor II|leuserpin 2|protease inhibitor leuserpin-2|serine (or cysteine) peptidase inhibitor, clade D, member 1|serine (or cysteine) proteinase inhibitor, clade D, member 1|serpin D1|serpin peptidase inhibitor, clade D (heparin cofactor), member 1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Serpind1, check out the Serpind1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Serpind1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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