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Product Info Summary
| SKU: | EK1937 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin). |
Product info
Product Name
Rat SMOC1 ELISA Kit PicoKine®
SKU/Catalog Number
EK1937
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat SMOC1 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Smoc1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat SMOC1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1937)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: H26-V452
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1937 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1937 is reactive to Smoc1 in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
Application Guarantee
EK1937 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of SMOC-1
This gene is mapped to 6q24. Human homolog binds calcium and may play a role in the extracellular matrix.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1937-CAP | Anti-Rat Smoc1 Pre-coated 96-well strip microplate | 1 |
| EK1937-ST | Rat Smoc1 Standard | 2 vials, 10 ng/tube |
| EK1937-DA | Rat Smoc1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.104 | 0.148 | 0.189 | 0.261 | 0.456 | 0.762 | 1.223 | 1.982 |
Data Example Images
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Rat SMOC1 PicoKine ELISA Kitstandard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect SMOC-1, Dilution ratio of 1:1, concentration in serum and plasma is around 2 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat SMOC1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 159 | 688 | 1452 | 168 | 675 | 1526 |
| Standard deviation | 7.63 | 39.22 | 66.79 | 8.57 | 39.83 | 97.66 |
| CV (%) | 4.8% | 5.7% | 4.6% | 5.1% | 5.9% | 6.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of SMOC-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 159 | 162 | 177 | 168 | 167 | 5.49 | 3.3% |
| Sample 2 | 688 | 757 | 693 | 684 | 706 | 14.11 | 2.0% |
| Sample 3 | 1452 | 1596 | 1441 | 1365 | 1464 | 83.42 | 5.7% |
Protein Target Info & Infographic
Gene/Protein Information For Smoc1 (Source: Uniprot.Org, NCBI)
Gene Name
Smoc1
Full Name
SPARC-related modular calcium-binding protein 1
Weight
51.076kDa
Alternative Names
Secreted modular calcium-binding protein 1; SMOC1; SMOC-1; SPARC related modular calcium binding 1; SPARC-related modular calcium-binding protein 1 Smoc1|2600002F22Rik, AI848508, S, SRG|SPARC related modular calcium binding 1|SPARC-related modular calcium-binding protein 1|SMOC-1|SPARC-related gene protein|SPARC-related protein|secreted modular calcium binding protein 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Smoc1, check out the Smoc1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Smoc1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat SMOC1 ELISA Kit PicoKine® (EK1937)
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No publications found for EK1937
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Rat SMOC1 ELISA Kit PicoKine®
Question
Q: Can SMOC-1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2020-10-28
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-10-28
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of SMOC-1 in Rat plasma? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Z. Clark
Verified customer
Asked: 2020-02-20
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the SMOC-1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-02-20
Question
Q: if the enzyme conjugated SMOC-1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the SMOC-1 antigen?
E. Carter
Verified customer
Asked: 2018-12-27
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-12-27
Question
Q: how can I thaw whole blood sample for SMOC-1 ELISA after freezing?
Z. Mangal
Verified customer
Asked: 2018-09-11
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test SMOC-1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-09-11
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
A. Williams
Verified customer
Asked: 2018-08-26
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-08-26
Question
Q: is it okay to use citrate plasma as samples in Rat SMOC-1 Picokine® ELISA Kit (Catalog # EK1937)?
L. Huang
Verified customer
Asked: 2016-09-16
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of SMOC-1 causing disruption of its protein structure. SMOC-1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the SMOC-1 ELISA, treating samples with different anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2016-09-16
