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Product Info Summary
| SKU: | EK0514 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (EDTA) and urine. |
Product info
Product Name
Rat TGF Beta 1 ELISA Kit PicoKine®
SKU/Catalog Number
EK0514
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat TGF Beta 1 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Tgfb1 in cell culture supernatants, serum, plasma (EDTA) and urine. Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat TGF Beta 1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0514)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: CHO; Immunogen sequence: A279-S390
Sensitivity
<1 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0514 for more details
Cross-reactivity
There is cross-reactivity with TGFbeta2, TGFbeta3, TGFbeta5<1%.
Reactive Species
EK0514 is reactive to TGFB1 in Rat samples
Validated Sample Types
cell culture supernatants, serum, plasma (EDTA) and urine.
Application Guarantee
EK0514 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TGFB1
Transforming growth factor-beta1 (TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects. TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation. TGFbeta1 is also an important mediator of bone remodeling. TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation. TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion. TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide (beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. The standard product used in this kit is recombinant TGFbeta1 with the molecular mass of 25KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0514-CAP | Anti-Rat TGFB1 Pre-coated 96-well strip microplate | 1 |
| EK0514-ST | Rat TGFB1 Standard | 2 vials, 10 ng/tube |
| EK0514-DA | Rat TGFB1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.044 | 0.095 | 0.184 | 0.243 | 0.419 | 0.819 | 1.163 | 1.972 |
Data Example Images
Click image to see more details
Rat TGF beta 1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TGFB1, Dilution ratio of 1:500, concentration in serum and plasma is 24-85 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat TGF Beta 1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 27 | 161 | 431 | 28 | 156 | 422 |
| Standard deviation | 1.59 | 9.82 | 23.7 | 1.73 | 9.98 | 26.16 |
| CV (%) | 5.5% | 6.1% | 5.5% | 6.2% | 6.4% | 6.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of TGFB1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 27 | 31 | 29 | 32 | 29 | 1.92 | 6.6% |
| Sample 2 | 161 | 143 | 162 | 145 | 152 | 8.78 | 5.7% |
| Sample 3 | 431 | 413 | 424 | 455 | 430 | 15.4 | 3.5% |
Protein Target Info & Infographic
Gene/Protein Information For TGFB1 (Source: Uniprot.Org, NCBI)
Gene Name
TGFB1
Full Name
Transforming growth factor beta-1 proprotein
Weight
44.341kDa
Superfamily
TGF-beta family
Alternative Names
CED; DPD1; LAP; TGFB; TGFB1; TGF-beta 1, 2, 3; TGFbeta; transforming growth factor, beta 1 TGFB1 CED, DPD1, IBDIMDE, LAP, TGF-beta1, TGFB, TGFbeta transforming growth factor beta 1 transforming growth factor beta-1 proprotein|TGF-beta-1|latency-associated peptide|prepro-transforming growth factor beta-1|transforming growth factor beta1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TGFB1, check out the TGFB1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TGFB1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat TGF Beta 1 ELISA Kit PicoKine® (EK0514)
Hello CJ!
EK0514 has been cited in 92 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
3,4-dihydroxyphenylethanol suppresses irradiation-induced pulmonary fibrosis in adult rats
Aldosterone Up-regulates Production of Plasminogen Activator Inhibitor-1 by Renal Mesangial Cells
Benefical Therapeutic Effect of Chinese Herbal Xinji′Erkang formula on Hypertension-Induced Renal Injury in the 2-Kidney-1-Clip Hypertensive Rats
Role of CCR7 on dendritic cell‑mediated immune tolerance in the airways of allergy‑induced asthmatic rats
Calcium‑sensing receptor promotes high glucose‑induced myocardial fibrosis via upregulation of the TGF‑β1/Smads pathway in cardiac fibroblasts
Protective effects of oleanolic acid on oxidative stress and the expression of cytokines and collagen by the AKT/NF‑κB pathway in silicotic rats
Efficacies of rosiglitazone and retinoin on bleomycin‑induced pulmonary fibrosis in rats
LSKL peptide alleviates subarachnoid fibrosis and hydrocephalus by inhibiting TSP1-mediated TGF-β1 signaling activity following subarachnoid hemorrhage in rats
Effect of Oxymatrine on the TGFbeta-Smad signaling pathway in rats with CCl4-induced hepatic fibrosis
The Potassium SK Channel Activator NS309 Protects Against Experimental Traumatic Brain Injury Through Anti-Inflammatory and Immunomodulatory Mechanisms
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
20 Customer Q&As for Rat TGF Beta 1 ELISA Kit PicoKine®
Question
EK0514 is experiencing a two-week delay during shipment, do you think the product quality will be compromised?
Verified customer
Asked: 2022-04-26
Answer
The Rat TGF Beta 1 ELISA Kit PicoKine® (EK0514) performance might be affected due to the delay because the shipping temperature is unstable. Protein activity will decrease as time goes on, so you may get lower O.D. values. We suggest to run a pilot test to generate the standard curve only once receive the kit to see if it performs as expected. If the standard curve is good, please move forward with testing samples.
Boster Scientific Support
Answered: 2022-04-27
Question
Does 10% FBS or phenol red in complete cell culture media affect this assay?
Verified customer
Asked: 2020-12-17
Answer
10% FBS or phenol red in complete cell culture media affects assay using the Rat TGF Beta 1 ELISA Kit PicoKine™ (EK0514).
Boster Scientific Support
Answered: 2020-12-18
Question
Does EK0514 by any chance recognize the mouse version? If not, do you have a kit that would recognize both rat and mouse (or even human)?
Verified customer
Asked: 2020-12-17
Answer
Yes, the Rat TGF Beta 1 ELISA Kit PicoKine™ (EK0514) is able to recognize the mouse version. We haven't got any kit that could recognize both rat and mouse.
Boster Scientific Support
Answered: 2020-12-18
Question
What is the recipe for the color developing reagent (TMB) for EK0514? I spilled some and would like to make up for what is lost, so the entire kit does not get wasted.
Verified customer
Asked: 2020-12-17
Answer
Unfortunately, we cannot disclose the recipe of the color developing reagent (TMB) for the Rat TGF Beta 1 ELISA Kit PicoKine™ (EK0514), but we can provide a free bottle to the customer.
Boster Scientific Support
Answered: 2020-12-18
Question
Q: how to analyze ELISA data? I measured TGFB1 level in serum.
T. Young
Verified customer
Asked: 2020-04-12
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-04-12
Question
I purchased Rat TGF Beta 1 ELISA Kit PicoKine and I was wondering if this kit would detect active or inactive TGF beta 1.
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
The Rat TGF Beta 1 ELISA Kit PicoKine will detect both active and inactive form of TGF Beta 1. However it will not detect the LAP complex.
Boster Scientific Support
Answered: 2019-12-18
Question
We've received an inquiry regarding cat # EK0514 from our client The customer is interested in this product and would like to know more details. They would like to know what is the detectable form of the TGFb1 for this kit? And, does it detect active and inactive TGFb1, LAP associated, homodimer?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
EK0514 detects active TGFb1.
Boster Scientific Support
Answered: 2019-11-29
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification TGFB1 using the EK0514 Rat TGFB1 Picokine® ELISA Kit?
W. Surve
Verified customer
Asked: 2019-11-21
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2019-11-21
Question
What is the recommended dilution for rat serum samples using EK0514?
Verified customer
Asked: 2019-11-05
Answer
The recommended dilution for rat serum samples using the Rat TGF Beta 1 ELISA Kit PicoKine™ (EK0514) is 1:100.
Boster Scientific Support
Answered: 2019-11-05
Question
Is there a standardized total protein amount of samples that should be pipetted per well, so that it lies within the assay range of EK0418, EK0514 and EK0393? Is protein quantification needed?
Verified customer
Asked: 2019-11-05
Answer
For the Rat IL-10 ELISA Kit PicoKine™ (EK0418), Rat TGF Beta 1 ELISA Kit PicoKine™ (EK0514) and Rat IL-1 Beta ELISA Kit PicoKine™ (EK0393), add 100ul of diluted sample into each well. It is unnecessary to quantify the amount of total protein.
Boster Scientific Support
Answered: 2019-11-05
Question
Is it possible to use plasma with heparin in the analyses of the EK0514?
Verified customer
Asked: 2019-10-24
Answer
The Rat TGF Beta 1 ELISA Kit PicoKine EK0514 has not been tested with plasma samples with heparin. We cannot guarantee that it would work. It is suggested to run pilot tests to see if the kits would work on plasma samples with heparin if you would like to try.
Boster Scientific Support
Answered: 2019-10-24
Question
Q: if the enzyme conjugated TGFB1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TGFB1 antigen?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-07-25
Question
Q: What is the optimal O.D. value for TGFB1 ELISA kit? I performed your TGFB1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain TGFB1 even though the O.D. values are not very high?
S. Lewis
Verified customer
Asked: 2019-07-21
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TGFB1 ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of TGFB1 that can be declared/interpreted as positive by the above standard is 1pg/ml.
Boster Scientific Support
Answered: 2019-07-21
Question
Q: can you recommend the dilution ratio of serum samples for detection of TGFB1 in Rat serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-05-18
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TGFB1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-05-18
Question
Q: which procedure should I follow in order to thaw whole blood sample for TGFB1 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-12-01
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TGFB1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-12-01
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-04-17
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-04-17
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-04-05
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-04-05
Question
What is the standard reconstitution instructions for antibody EK0514 Rat TGF Beta 1 ELISA Kit PicoKine?
Verified Customer
Verified customer
Asked: 2017-08-09
Answer
The standard for EK0514 Rat TGF Beta 1 ELISA Kit PicoKine should be reconstituted in 400ul to a concentration of 400pg/ml.
Boster Scientific Support
Answered: 2017-08-09
Question
Our customer is very interested in this product. They have further questions about it. They would like to confirm the active form will have some cross over homology in several of the species of active and inactive TGFb1, LAP associated, homodimer. So can please give us more details about the specificity and any of the species it will binds and how it is that it only binds to the active protein species?
Verified Customer
Verified customer
Asked: 2015-05-22
Answer
EK0514 is able to detect active TGF beta 1 in activated human and mouse samples. And it has cross reactivity with
Boster Scientific Support
Answered: 2015-05-22
Question
What is your recommended protocol on preparing tissues (such as bone) for ELISA analysis? Keyword: sample preparation, treatment procedures
Verified Customer
Verified customer
Asked: 2013-11-25
Answer
The protocol for bone tissue is as follows - Bone Tissue: Extract demineralized bone samples in 4 M Guanidine-HCl and protease inhibitors. Dissolve the final sample in 2 M Guanidine-HCl. However, we did not test tissue samples with the Elisa kits so a pilot experiment is recommended.
Boster Scientific Support
Answered: 2013-11-25
