Human TGF Beta 1 ELISA Kit PicoKine®

Product Name

Human TGF Beta 1 ELISA Kit PicoKine®

SKU/Catalog Number

EK0513

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

TGF-β/SMAD signaling → regulation of fibrosis, epithelial–mesenchymal transition, and immune modulation; clinical utility (research): monitoring TGFB1 in fibrosis, tumor microenvironment, and immune regulation studies. Assay context: PicoKine® sandwich ELISA; human matrices include serum, EDTA plasma, urine, and cell culture supernatants; sensitivity <1 pg/mL; assay range 15.6–1,000 pg/mL; minimal cross-reactivity with TGF-β2/β3/β5 (<1%) (from product page). Often profiled alongside MMP-2 for ECM remodeling and HGF for growth/motility signaling; can be contrasted with pro-inflammatory cytokines such as IL-6 or TNF-α to contextualize immune milieu.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human TGF Beta 1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0513)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: CHO; Immunogen sequence: A279-S390

Sensitivity

<1 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Standard Dilution Instructions

See datasheet of EK0513 for more details

Cross-reactivity

There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1%.

Reactive Species

EK0513 is reactive to TGFB1 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (EDTA) and urine.

Application Guarantee

EK0513 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of TGFB1

Transforming growth factor-beta1 (TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects.TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation.TGFbeta1 is also an important mediator of bone remodeling.TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation.TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion. TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide (beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. The standard product used in this kit is recombinant TGFbeta1 with the molecular mass of 25KDa.

Kit Components

Catalog Number	Description	Quantity
EK0513-CAP	Anti-Human TGFB1 Pre-coated 96-well strip microplate	1
EK0513-ST	Human TGFB1 Standard	2 vials, 10 ng/tube
EK0513-DA	Human TGFB1 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.011	0.154	0.256	0.452	0.734	1.221	1.878	2.684

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect TGFB1, For serum, dilution ratio is 1:200 and concentration is 14631-76099 pg/ml.
For plasma, dilution ratio is 1:200 and concentration is 722-1984 pg/ml..

Some articles we found to cite concentrations of TGFB1 in samples: 21786325 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human TGF Beta 1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

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